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The role of TRPC6 in HGF-induced cell proliferation of humanprostate cancer DU145 and PC3 cells

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Wang Yong 1   Yue Dan 2   Li Kai 3   Liu Yili 1   Ren Changshan 4   Wang Ping 1  
文摘 Hepatocyte growth factor (HGF) is a glycoprotein that induces prostate cancer cell proliferation, migration and invasion. The activation of transient receptor potential canonical 6 (TRPC6) channels is considered important inpromoting prostate cancer cell proliferation. In this study, we assessed the role of endogenous TRPC6 channels inthe HGF-induced cell proliferation of prostate cancer. Reverse transcription-PCR and Western blotting were usedto investigate TRPC6 expression. Electrophysiological techniques (whole-cell patch clamp configuration) and Ca~(2+) imaging analysis were used to investigate the channel activity in cells. The effects of TRPC6 channels on cell cycleprogression, cell apoptosis and cell growth were also examined. TRPC6 and c-MET were expressed in DU145 andPC3 cells. In addition, functional TRPC6 channels were present in DU145 and PC3 cells, and TRPC6 knockdownsuppressed TRPC-like currents evoked by oleoyl-2-acetyl-sn-glycerol (OAG). Inhibition of TRPC6 channels in DU145 and PC3 cells abolished OAG- and HGF-induced Ca~(2+) entry. Furthermore, inhibition of TRPC6 channels arrested DU145 and PC3 cells at the G_2/M phase and suppressed HGF-induced cell proliferation. Collectively, ourresults indicate that TRPC6 has an important role in HGF-induced DU145 and PC3 cell proliferation
来源 Asian Journal of Andrology ,2010,12(6):841-852 【核心库】
DOI 10.1038/aja.2010.85
关键词 hepatocyte growth factor ; human prostate cancer ; proliferation ; TRPC6
地址

1. Department of Urology, Fourth Affiliated Hospital, China Medical University, Shenyang, 110032  

2. College of Medical Laboratory Science, Tianjin Medical University, Tianjin, 300070  

3. Department of Surgical Oncology, First Affiliated Hospital, China Medical University, Shenyang, 110001  

4. Cancer Research Institute, First Affiliated Hospital, China Medical University, Shenyang, 110001

语种 英文
文献类型 研究性论文
ISSN 1008-682X
学科 特种医学
文献收藏号 CSCD:4073832

参考文献 共 29 共2页

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引证文献 1 篇

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