小叶锦鸡儿根际微生物群落功能多样性对环境变化的响应
Response of microbial community functional dive RSity in the rhizosphere of Caragana microphylla to environmental change
查看参考文献40篇
|
文摘
|
利用Biolog技术对内蒙古草原灌丛优势种小叶锦鸡儿(Caragana microphylla)根际土壤微生物群落功能多样性特征及其对大气CO_2浓度、土壤氮水平和土壤水分3个环境因子变化的响应进行了研究. 结果表明:(1)小叶锦鸡儿根际土壤微生物利用碳源总量在整个培养过程中呈逐渐增加的趋势. 其利用比例较高的碳源类型为聚合物、糖类和氨基酸. (2)主成分分析表明, 8个处理组的微生物群落功能多样性差异显著, 其中与主成分1显著相关的碳源有14种, 分别属于聚合物、糖类、氨基酸和羧酸. (3)加倍CO_2浓度极显著提高平均颜色变化率(AWCD)以及丰富度指数和Shannon均匀度. (4)氮素添加使AWCD、丰富度指数和Shannon均匀度均极显著降低, 其抑制效应在加倍CO_2浓度时有所缓解. (5)加水处理对上述指标均有一定的促进作用, 但是差异未达显著水平. (6)加倍CO_2浓度和氮素添加联合处理下, 小叶锦鸡儿根际微生物活性高于对照处理, 说明加倍CO_2浓度对微生物活性的促进效应强于添加氮素的抑制效应. (7)CO_2和氮素对上述指标有交互作用. 综上所述, 小叶锦鸡儿根际土壤微生物群落的功能在很大程度上受到外界环境因子的影响, 对环境变化较敏感的碳源类型为聚合物、糖类、氨基酸和羧酸, 与利用比例较高的碳源类型基本一致 |
|
其他语种文摘
|
Functional dive RSity of soil microbial communities in rhizosphere of Caragana microphylla, a dominant shrubby leguminous species in the Inner Mongolia Grassland Ecosystem, was examined using the Biolog method to evaluate the effects of environmental changes in atmospheric CO_2 concentration, soil nitrogen level and water level on soil microbial community. The results showed that total amount of carbon sources utilized by microbes in rhizosphere of C. microphylla increased with the incubation time. The main types of carbon sources utilized were polyme RS, carbohydrates and amino acids. Principal Component Analysis indicated that microbial community functional dive RSity varied significantly among 8 treatment groups. Fourteen categories of carbon sources were significantly related to the principal component 1, which belonged to polyme RS, carbohydrates, amino acids, and carboxylic acids. Elevated CO_2 significantly increased the value of average well colour development (AWCD), richness index and Shannon evenness. Under nitrogen addition treatments, AWCD, richness index and Shannon evenness were significantly decreased, but this inhibition was slightly alleviated under elevated CO_2. Water addition showed no significant effect on AWCD and dive RSity indices of soil microbial community. Rhizospheric microbial activity were higher under combined treatments of elevated CO_2 and nitrogen addition than CK, which implied that the positive effect of elevated CO_2 on microbial activity of C. microphylla was larger than the negative effect of nitrogen addition on it. There was significant CO_2 nitrogen interaction on AWCD, richness index and Shannon evenness. In conclusion, microbial community functional dive RSity in rhizosphere of C. microphylla was sensitive to environmental changes, with the sensitive carbon source types being polyme RS, carbohydrates, amino acids, and carboxylic acids, essentially those carbon sources that are also the most heavily used |
|
来源
|
生态学报
,2010,30(12):3087-3097 【核心库】
|
|
关键词
|
小叶锦鸡儿
;
Biolog
;
土壤微生物
;
多样性
;
CO_2
;
氮素
;
水分
|
|
地址
|
中国科学院植物研究所, 植被与环境变化国家重点实验室, 北京, 100093
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1000-0933 |
|
学科
|
普通生物学 |
|
基金
|
国家自然科学基金资助项目
;
中国科学院知识创新工程重要方向项目
;
中国科学院野外台站基金项目
;
中国科学院植物研究所植被与环境变化国家重点实验室第五自主课题资助项目
|
|
文献收藏号
|
CSCD:3948944
|
参考文献 共
40
共2页
|
|
1.
Clark F E. The microflora of grassland.
Advances in Agronomy,1970,22:375-435
|
被引
27
次
|
|
|
|
|
2.
Erb R W. Detection of polychlorinated biphenyl degradation genes in polluted sediments by direct DNA extraction and polymerase chain reaction.
Applied and Environmental Microbiology,1993,59(12):4065-4073
|
被引
15
次
|
|
|
|
|
3.
Johnsen K. Pesticide effects on bacterial dive RSity in agricultural soils.
Biology and Fertility of Soils,2001,33(6):443-453
|
被引
40
次
|
|
|
|
|
4.
林文雄. 水稻化感作用及其分子生态学研究进展.
生态学报,2006,26(8):2687-2694
|
被引
47
次
|
|
|
|
|
5.
林瑞余. 苗期化感水稻对根际土壤微生物群落及其功能多样性的影响.
生态学报,2007,27(9):3644-3654
|
被引
37
次
|
|
|
|
|
6.
Garland J L. Analytical approaches to the characterization of samples of microbial communities using patterns of potential C source utilization.
Soil Biology and Biochemistry,1996,28(2):213-221
|
被引
115
次
|
|
|
|
|
7.
Gilbride K A. Phenotypic fingerprinting of microbial communities in wastewater treatment systems.
Water Research,1996,30(5):1077-1086
|
被引
2
次
|
|
|
|
|
8.
Ibekwe A M. Phospholipid fatty acid profiles and carbon utilization patterns for analysis of microbial community structure under field and greenhouse conditions.
FEMS Microbiology Ecology,1998,26(2):151-163
|
被引
30
次
|
|
|
|
|
9.
IPCC. Climate Chang2007: The Physical Science Basis.
Working Group I Contribution to the Fourth Assessment Report of the IPCC,2007:1-21
|
被引
1
次
|
|
|
|
|
10.
Kao-Kniffin J. Elevated CO_2 differentially alte RS belowground plant and soil microbial community structure in reed canary grass-invaded experimental wetlands.
Soil Biology and Biochemistry,2007,39(2):517-525
|
被引
5
次
|
|
|
|
|
11.
Montealegre C M. Changes in microbial activity and composition in a pasture ecosystem exposed to elevated atmospheric carbon dioxide.
Plant and Soil,2002,243(2):197-207
|
被引
20
次
|
|
|
|
|
12.
Kandeler E. Response of soil microbial biomass and enzyme activities to the transient elevation of carbon dioxide in a semi-arid grassland.
Soil Biology and Biochemistry,2006,38(8):2448-2460
|
被引
15
次
|
|
|
|
|
13.
Ronn R. Response of free-living soil protozoa and microorganisms to elevated atmospheric CO_2 and presence of mycorrhiza.
Soil Biology and Biochemistry,2002,34(7):923-932
|
被引
18
次
|
|
|
|
|
14.
李杨. 大气CO_2浓度升高对农田土壤微生物及其相关因素的影响.
应用生态学报,2003,14(12):2321-2325
|
被引
25
次
|
|
|
|
|
15.
Lee B J A. Ecological effects of atmospheric reactive nitrogen deposition on semi-natural terrestrial ecosystems.
New Phytologist,1998,139(1):127-134
|
被引
1
次
|
|
|
|
|
16.
Kemmitt S J. pH regulation of carbon and nitrogen dynamics in two agricultural soils.
Soil Biology and Biochemistry,2006,38(5):898-911
|
被引
79
次
|
|
|
|
|
17.
Wang A S. Changes in soil biological activities under reduced soil pH during Thlaspi caerulescens phytoextraction.
Soil Biology and Biochemistry,2006,38(6):1451-1461
|
被引
20
次
|
|
|
|
|
18.
Drenovsky R E. Soil water content and organic carbon availability are major determinants of soil microbial community composition.
Microbial Ecology,2004,48(3):424-430
|
被引
62
次
|
|
|
|
|
19.
刘岳燕. 水分条件对水稻土微生物群落多样性及活性的影响.
土壤学报,2006,43(5):828-834
|
被引
38
次
|
|
|
|
|
20.
Bloor J M G. CO_2 and inorganic N supply modify competition for N between co-occurring grass plants, tree seedlings and soil microorganisms.
Soil Biology and Biochemistry,2009,41(3):544-552
|
被引
8
次
|
|
|
|
|
了解气象卫星更多信息，请访问