四膜虫omc基因的克隆及在滴滴涕(DDT)暴露下的表达分析
Cloning and Expression Analysis of Tetrahymena 2-oxoglutarate/Malate Carrier Gene (omc) Exposed to Dichlorodiphenyltrichloroethane (DDT)
查看参考文献28篇
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文摘
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针对环境激素类污染物滴滴涕(DDT)危害大和分子机理复杂等问题, 以之前筛选出的一个DDT暴露下差异表达的基因一一酮戊二酸/苹果酸载体蛋白基因(2-oxoglutarate/malate carrier, omc)为研究对象, 克隆得到该基因转录mRNA的全长为1 060 bp, 其中5'、3'非翻译区分别为46 bp和99 bp;实时定量PCR方法测定不同浓度DDT暴露下该基因的表达水平下降了20%~50%.四膜虫omc基因上游数个AP-1和NF-kappa B位点的存在, 提示该基因表达受DDT调控的模式可能是与高等动物类似的雌激素受体间接调控途径; 同时根据omc基因存在多个与三羧酸循环和电子传递链相关的共表达基因的结果推测, DDT可能通过调控omc等基因来影响线粒体正常功能, 进而对细胞产生毒害作用.图3表1参28 |
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其他语种文摘
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As a kind of environmental hormones, dichlorodiphenyltrichloroethane (DDT) is harmful and its molecular mechanism is unclear. In this study, Tetrahymena thermophila 2-oxoglutarate/malate carrier gene (omc), which was down-regulated with DDT treatment, was cloned and sequenced. Its complete mRNA was 1 060 bp with a 46-bp 5'-untranslated region (UTR) and a 99-bp 3' UTR. At different concentrations of DDT, it was down-regulated by 20%~50% that was detected by real-time quantitative PCR. Several AP-1 and NF-Kappa B factors found in T. thermophila omc upstream region indicated that the regulatory mode of DDT to omc was likely the indirect regulation of estrogen receptor in mammalian. At the same time, the coexpressed genes with omc in T. thermophila were involved in the tricarboxylic acid cycle and electron-transport chain, which suggested that DDT probably inhibited the function of mitochondria by regulating these genes, and then did harm to the cells. Fig 3, Tab l, Ref 28 |
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来源
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应用与环境生物学报
,2010,16(2):216-221 【核心库】
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DOI
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10.3724/sp.j.1145.2010.00216
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关键词
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四膜虫
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omc
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DDT
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表达
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调控
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地址
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中国科学院水生生物研究所, 武汉, 430072
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1006-687X |
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学科
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分子生物学;环境科学基础理论 |
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基金
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国家科技部国际合作项目
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湖北省武汉市青年科技晨光计划项目
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文献收藏号
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CSCD:3938577
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