成团泛菌MFC-3的分离鉴定及其腐殖质/Fe(Ⅲ)呼吸特性研究
Isolation and Characterization of a Facultative AnaerobePantoea agglomerans MFC-3 and Its Humic Substance-and Fe(Ⅲ)-Respiring Activity
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文摘
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从地下古森林沉积物样品中富集分离到1株腐殖质/Fe(Ⅲ)还原菌MFC-3菌株,经16S rDNA基因序列分析,该菌与成团泛菌Pantoea agglomeransWAB1951的相似性为99%,确定为成团泛菌.通过序批式厌氧实验考察了MFC-3的腐殖质呼吸活性、电子利用情况以及对4种铁氧化物的还原活性.结果表明, MFC-3能够以AQDS为唯一电子受体进行厌氧胞外呼吸,可利用的电子供体有:甲酸、乳酸、丙三醇、柠檬酸、葡萄糖和蔗糖,且AQDS还原速率顺序为:蔗糖>葡萄糖>柠檬酸>乳酸>丙三醇>甲酸;以葡萄糖作为电子供体时,48 h内0.3 mmol·L~(-1)的AQDS被还原,同时4.5 mmol·L~(-1)葡萄糖被消耗,菌数增殖近7倍,证明MFC-3能够进行腐殖质呼吸;MFC-3还能以多种Fe(Ⅲ)氧化物为电子受体进行厌氧呼吸,25 d内分别有2.5 mmol·L~(-1)水铁矿、2.1 mmol·L~(-1)纤铁矿、2.3 mmol·L~(-1)针铁矿及0.8 mmol·L~(-1)赤铁矿被还原溶解.本研究为胞外呼吸研究与应用提供1株适宜的模式菌株 |
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其他语种文摘
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A strain of humic substance-and Fe(Ⅲ)-reducing bacterium was isolated from the subterranean forest sediment and designated as MFC-3.The strain is facultative anaerobic, Gram-negative, motile and rod (1.0-3.0μm long, 0.5-1.0μmwide) and identified asPantoea agglomeranswiththe 16S rDNAsequence analyses. Batch experimentswere conducted to investigate its humic substance-and Fe(Ⅲ)-respiring activity. The results showed thatMFC-3 was capable of anaerobic respiration on anthraquinone-2,6-disulphonate (AQDS) as the sole terminal electron acceptor with glucose as the electron donor. Within 48 h, MFC-3 could reduce 0.3 mmol·L~(-1)AQDS atthe expense of 4.5 mmol·L~(-1) glucose, and the population of bacteria was increased by 7 times. The strain could use sucrose, glucose, citrate, lactate and formate as electron donors for anaerobic respiration, and the reduction rates of AQDS ranked as sucrose (77%)> glucose (66%) > citrate (50%) > lactate (33%) > glycerol (25%)> formate (17%). MFC-3 can also effectively reduce four types of Fe(Ⅲ) oxides. After 25 d, the total Fe(Ⅱ) concentration in the tests of using ferrihydrite,α-FeOOH,γ-FeOOHorα-Fe_2O_3 as electron acceptor reached 2.5, 2.1, 2.3 and 0.8 mmol·L~(-1), respectively. As a strain of environmental origin, MFC-3 is quite useful forthe study of extracellular respiration and bioremediation of chlorinated organic pollutants in Fe(Ⅲ)/humic substance-rich environments |
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来源
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环境科学
,2010,31(1):237-242 【核心库】
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关键词
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成团泛菌
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腐殖质
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铁氧化物
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腐殖质/Fe(Ⅲ)呼吸
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胞外呼吸
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地址
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1.
中国科学院广州地球化学研究所, 广州, 510640
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广东省生态环境与土壤研究所, 广东省农业环境综合治理重点实验室, 广州, 510650
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0250-3301 |
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学科
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环境科学基础理论 |
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基金
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国家自然科学基金项目
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广东省自然科学基金
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文献收藏号
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CSCD:3933847
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