应用16S rDNA克隆文库技术研究长期稻草还田对水稻土细菌多样性的影响
Effect of long-term fertilization by the application of rice straw on bacterial diversity in paddy soil
查看参考文献46篇
|
文摘
|
利用中国科学院桃源农业生态试验站施肥制度长期定位试验田对照(CK)和稻草还田(OM)施肥处理的土壤样品, 应用 16S rDNA克隆文库技术直接提取土壤微生物总DNA, 分别构建细菌16S rDNA克隆文库, 并进行序列测定和分析. 结果表明, 与对照(CK)相比, 稻草还田(OM)后土壤细菌群落结构发生了显著改变, 土壤细菌多样性和均匀度均有所降低. 对照(CK)和稻草还田(OM)两个施肥处理的优势种群均为变形菌, 酸杆菌次之;稻草还田减少了变形菌、疣微菌、绿弯菌和绿菌的分布, 而增加了硝化螺旋菌的分布. 16S rDNA系统发育分析则表明, 稻草还田对酸杆菌群落结构影响最大, 其次是疣微菌和δ-变形菌. |
|
其他语种文摘
|
Paddy soil bacterial community structure was studied through 16S rDNA gene. The soil samples were collected from the long-term fertilization experiment field at the Taoyuan Agro-ecosystem Research Station. Total microbial DNA was directly extracted from both treatments including amended with rice straw ( OM) and CK. The clone libraries were constructed after cloning and sequencing. The result showed that the bacterial communtiy composition of the paddy soil amended with rice straw (OM) was changed significantly compared with CK, both Shannon index and evenness decreased indicating its bacterial diversity was decreased. Proteobacteria were predominated both in rice straw ( OM) and CK treatments, followed by Acidobacteria. Application of rice straw induced obvious decrease of the genotypes of Proteobacteria, Verrucomicrobia, Chloroflexi and Chlorobi compared to CK, in the contrast, the genotypes of Nitrospira increased. The phylogenetic tree based on the 16S rDNA sequences showed that application of rice straw varied the distribution of Acidobacteria greatest, followed by Verrucomicrobia and Delta-Proteobacteria. Therefore, application of rice straw had an important impact on bacterial community structure. |
|
来源
|
生态学报
,2010,30(14):3865-3874 【核心库】
|
|
关键词
|
16S rDNA
;
水稻土
;
长期施肥
;
细菌多样性
;
系统发育分析
|
|
地址
|
中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 湖南, 410125
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1000-0933 |
|
学科
|
普通生物学 |
|
基金
|
国家自然科学基金资助项目
;
中国科学院“百人计划”项目
;
国家科技支撑计划项目
|
|
文献收藏号
|
CSCD:3913912
|
参考文献 共
46
共3页
|
|
1.
区惠平. 稻草还田在水稻生产上的应用与研究进展.
作物杂志,2007(6):9-11
|
被引
7
次
|
|
|
|
|
2.
周江明. 稻草还田综合效益研究.
中国农学通报,2002,18(4):7-10
|
被引
38
次
|
|
|
|
|
3.
邱孝煊. 稻草还田对红壤性水稻土肥力的影响.
土壤肥料科学,2006,22(1):188-190
|
被引
2
次
|
|
|
|
|
4.
佘冬立. 稻草还田的土壤肥力与产量效应研究.
中国生态农业学报,2008,16(1):100-104
|
被引
15
次
|
|
|
|
|
5.
庞新. 不同供氮水平对根际微生物量氮及微生物活度的影响.
植物营养与肥料学报,2000,10(4):476-480
|
被引
21
次
|
|
|
|
|
6.
Collins H P. Crop rotation and residue management effects on soil carbon and microbial dynamics.
Soil Science Society of America,1992,56:783-788
|
被引
26
次
|
|
|
|
|
7.
Kent A D. Microbial communities and their interactions in soil and rhizosphere ecosystems.
Annual Review of Microbiology,2002,56(10):211-236
|
被引
25
次
|
|
|
|
|
8.
Dawson R C. Effect of crop residues on soil micropopulations, aggregation, and fertility under Maryland condition.
Soil Science Society of America Proceedings,1946,10:180-184
|
被引
1
次
|
|
|
|
|
9.
周文新. 稻草还田对土壤微生物群落功能多样性的影响.
环境科学学报,2008,28(2):326-330
|
被引
35
次
|
|
|
|
|
10.
陈秀蓉. 细菌多样性及其在农业生态系统中的作用.
草业科学,2002,19(9):34-38
|
被引
29
次
|
|
|
|
|
11.
Ward D M. 16S rRNA sequences reveal numerous uncultured microorganisms in a nature community.
Nature,1990,345(6270):63-65
|
被引
89
次
|
|
|
|
|
12.
谭周进. 稻草还田量对晚稻土微生物数量及活度的动态影响.
农业环境科学学报,2006,25(3):670-673
|
被引
31
次
|
|
|
|
|
13.
Arcate J M. Diversity of peronosporomycete (oomycete) communities associated with the rhizosphere of different plant species.
Microbial Ecology,2006,51(1):36-50
|
被引
1
次
|
|
|
|
|
14.
Saleena L M. Diversity of Azospirillum strains isolated from rice plants grown in saline and nonsaline sites of coastal agricultural ecosystem.
Microbial Ecology,2002,44(3):271-277
|
被引
3
次
|
|
|
|
|
15.
张建萍. 应用16SrDNA-RFLP方法分析宁夏地区稻田土壤细菌的多样性.
生物多样性,2008,16(6):586-592
|
被引
16
次
|
|
|
|
|
16.
刘玮琦. 应用16S rRNA基因文库技术分析土壤细菌群落的多样性.
微生物学报,2008,48(10):1344-1350
|
被引
52
次
|
|
|
|
|
17.
Proteous L A. An effective method to extract DNA from environmental samples for polymerase chain reaction amplification and DNA fingerprint analysis.
Curr Microbiol,1994,29(5):301-307
|
被引
19
次
|
|
|
|
|
18.
杨建. 红树林土壤总DNA不同提取方法比较研究.
生物技术通报,2006(增刊):366-371
|
被引
17
次
|
|
|
|
|
19.
Cole J R. The Ribosomal Database Project (RDP-II) : previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy.
Nucleic Acids Research,2003,31(1):442-443
|
被引
34
次
|
|
|
|
|
20.
Kowalchuk G A. Application of ecological diversity statistics in microbial ecology.
Molecular Microbial Ecology Manual, 2nd ed,2004:1321-1344
|
被引
1
次
|
|
|
|
|
了解气象卫星更多信息，请访问