乙肝表面抗原在微球表面的吸附行为、活性和结构变化
Adsorption Behavior, Bioactivity and Structure of HBsAg on Surface of PLA Microspheres
查看参考文献23篇
文摘
|
采用快速膜乳化技术结合溶剂挥发法制备了尺寸均一的聚乳酸(PLA)微球, 平均粒径为800 nm左右, 并采用 PLA微球对乙肝表面抗原(HBsAg)进行了吸附研究, 考察了pH值、盐浓度、微球用量、HBsAg浓度及吸附温度对 HBsAg吸附率、活性和结构的影响.结果表明, 在pH 6.0的磷酸缓冲溶液中, NaCl浓度为20 g/L、微球用量为20 mg/mL、HBsAg浓度为10μg/mL、吸附温度为37℃的条件下, HBsAg吸附率可达60%左右.4℃下, pH值为8.0及 NaCl浓度为1 g/L、微球用量为2 mg/mL及HBsAg浓度为300μg/mL时, HBsAg活性保留可达98%以上. |
其他语种文摘
|
Relatively uniform-sized poly(lactic acid)(PLA)microspheres with the average particle size of 800 nm were successfully prepared by premix membrane emulsification combined with solvent evaporation method. The effects of pH value, salt concentration, amount of PLA microspheres, HBsAg concentration and adsorption temperature on the adsorption efficiency, bioactivity retention and structure of HBsAg were systemically investigated. The results showed that the adsorption efficiency of HBsAg on the surface of PLA microspheres could reach more than 60%under the conditions of pH 6.0 phosphate buffer, 20 g/L NaCl concentration, 20 mg/mL PLA microspheres, 10μg/mL HBsAg concentration and 37℃adsorption temperature. The bioactivity retention of HBsAg exceeded 98% under 4℃with the following conditions:pH value of phosphate buffer at 8.0, concentration of NaCl 1 g/L, amount of microspheres 2 mg/mL and initial HBsAg concentration 300μg/mL. |
来源
|
过程工程学报
,2010,10(3):559-567 【核心库】
|
关键词
|
聚乳酸微球
;
乙肝表面抗原
;
吸附
;
生物活性
;
结构
|
地址
|
1.
中国科学院研究生院, 生化工程国家重点实验室, 北京, 100049
2.
中国科学院过程工程研究所, 生化工程国家重点实验室, 北京, 100190
3.
北京化工大学生命科学与技术学院, 生化工程国家重点实验室, 北京, 100029
|
语种
|
中文 |
文献类型
|
研究性论文 |
ISSN
|
1009-606X |
学科
|
药学 |
基金
|
国家自然科学基金国家杰出青年科学基金
;
国家863计划
|
文献收藏号
|
CSCD:3912438
|
参考文献 共
23
共2页
|
1.
Couvreur P. Multiple Emulsion Technology for the Design of Microspheres Containing Peptides and Oligopeptides.
Adv. Drug Del.Rev,1997,28(1):85-87
|
被引
7
次
|
|
|
|
2.
Perugini P. Study on Glycolic Acid Delivery by Liposomes and Microspheres.
Int.J.Pharm,2000,196(1):51-61
|
被引
9
次
|
|
|
|
3.
田瑞. 快速膜乳化法制备粒径均一的PLGA微球和微囊.
过程工程学报,2009,9(4):754-762
|
被引
13
次
|
|
|
|
4.
Jintapattanakit A. Peroral Delivery of Insulin Using Chitosan Derivatives:A Comparative Study of Polyelectrolyte Nanocomplexes and Nanoparticles.
Int.J. Pharm,2007,342(1/2):240-249
|
被引
4
次
|
|
|
|
5.
Kamei N. Permeation Characteristics of Oligoarginine through Intestinal Epithelium and Its Usefulness for Intestinal Peptide Drug Delivery.
J.Controlled Release,2008,131(2):94-99
|
被引
4
次
|
|
|
|
6.
Russ V. Oligoethylenimine-grafted Polypropylenimine Dendrimers as Degradable and Biocompatible Synthetic Vectors for Gene Delivery.
J.Controlled Release,2008,132(2):131-140
|
被引
4
次
|
|
|
|
7.
Putney S D. Encapsulation of Proteins for Improved Delivery.
Curr. Opin. Chem.Biol,1998,2(4):548-552
|
被引
3
次
|
|
|
|
8.
Miyata T. Biomolecule-sensitive Hydrogels.
Adv. Drug Del. Rev,2002,54(1):79-98
|
被引
27
次
|
|
|
|
9.
Vila A. Design of Biodegradable Particles for Protein Delivery.
J.Controlled Release,2002,78(1):15-24
|
被引
39
次
|
|
|
|
10.
Li X H. Poly-DL-lactide-poly-(ethylene glycol) Microspheres as Oral and Parenteral Delivery Systems for Hepatitis B Antigen.
J.Appl. Polym.Sci,2002,83(4):850-856
|
被引
2
次
|
|
|
|
11.
Weissenb-ck A. WGA-grafted PLGA Nanospheres:Preparation and Association with Caco-2 Single Cells.
J.Controlled Release,2004,99(3):383-392
|
被引
1
次
|
|
|
|
12.
Li Y. Characterization of the Large Size Aggregation of Hepatitis B Virus Surface Antigen(HBsAg)Formed in Ultrafiltration Process.
Process Biochem,2007,42(3):315-319
|
被引
1
次
|
|
|
|
13.
Zhou W B. Molecular Characterization of Recombinant Hepatitis B Surface Antigen from Chinese Hamster Ovary and Hansenula Polymorpha Cells by High-performance Size Exclusion Chromatography and Multi-angle Laser Light Scattering.
J.Chromatogr.B,2006,838(2):71-77
|
被引
3
次
|
|
|
|
14.
Dreesman G R. Biophysical and Biochemical Heterogeneity of Purified Hepatitis B Antigen.
J. Virol,1972,10(3):469-476
|
被引
4
次
|
|
|
|
15.
Diminsky D. Physical, Chemical and Immunological Stability of CHO-derived Hepatitis B Surface Antigen Particles.
Vaccine,2000,18(1/2):3-17
|
被引
1
次
|
|
|
|
16.
Tleugabulova D. Aggregation of Recombinant Hepatitis B Surface Antigen in Pichia pastoris.
J.Chromatogr.B,1998,716(1/2):209-219
|
被引
1
次
|
|
|
|
17.
Anderson J M. Biodegradation and Biocompatibility of PLA and PLGA Microspheres.
Adv. Drug Del. Rev,1997,28(1):5-24
|
被引
88
次
|
|
|
|
18.
Tamber H. Formulation Aspects of Biodegradable Polymeric Microspheres for Antigen Delivery.
Adv. Drug Del.Rev,2005,57(3):357-376
|
被引
14
次
|
|
|
|
19.
Wei Q. Uniform-sized PLA Nanoparticles:Preparation by Premix Membrane Emulsification.
Int.J.Pharm,2008,359(1):294-297
|
被引
6
次
|
|
|
|
20.
Pace C N. Forces Contributing to the Conformational Stability of Proteins.
FASEB J,1996,10(1):75-83
|
被引
8
次
|
|
|
|
|