帮助 关于我们

返回检索结果

Immunological rejection of acellular heterogeneous nerve transplant for bridging the sciatic nerve in rats

查看参考文献26篇

文摘 BACKGROUND: Artificial materials composed of acellular heterogeneous nerves can resolve donor shortage problems for the repair of peripheral nerve defects. However, it remains unclear whether artificial materials can overcome immunological rejection of heterogeneous nerve grafts and obtain similar effects as allogeneic nerve grafts. OBJECTIVE: To analyze regeneration and immunological rejection of defective sciatic nerves in rats through the use of acellular heterogeneous nerve grafts. DESIGN, TIME AND SETTING: A randomized, controlled study was performed at the Department of Anatomy, China Medical University and the Experimental Center, First Affiliated Hospital, China Medical University between January and December 2008. MATERIALS: TritonX-100 (Sigma, USA) and deoxycholate (Pierce, USA) were used. METHODS: Bilateral sciatic nerves were collected from adult rabbits and treated with TritonX-100 and sodium deoxycholate to prepare acellular sciatic nerves, which were used to bridge 1-cm defective sciatic nerves in adult rats. MAIN OUTCOME MEASURES: The lymphocyte percentage in leukocytes was quantified following hemocyte staining. Neural regeneration and the recovery of motor end plates in the gastrocnemius muscle were observed under optical and electronic microscopy following toluidine blue staining, as well as acetylcholinesterase and succinate dehydrogenase histochemical staining. RESULTS: There was no significant difference in the lymphocyte percentage in leucocytes between transplanted and normal rats (P < 0.05). At 3 months after surgery, the rat toes on the operated side were separated and the rats could walk. In addition, the footplates exhibited an escape response when acupunctured. A large number of regenerated nerve fibers were observed in the transplant group, and acetylcholinesterase-positive motor end plates were visible in fibers of the gastrocnemius muscle. CONCLUSION: Acellular heterogeneous nerve transplants for the repair of defective sciatic nerves in rats promote neural regeneration without significant immunological rejection.
来源 Neural Regeneration Research ,2010,5(9):673-677 【核心库】
DOI 10.3969/j.issn.1673-5374.2010.09.006
关键词 heterogeneous nerve transplant ; peripheral nerve defect ; immunological rejection ; sciatic nerve ; neural regeneration
地址

1. Department of Neurosurgery, First Affiliated Hospital, China Medical University, Liaoning, Shenyang, 110001  

2. Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Liaoning, Shenyang, 110032  

3. Department of Human Anatomy, Basic College of Medical Sciences, China Medical University, Liaoning, Shenyang, 110001

语种 英文
文献类型 研究性论文
ISSN 1673-5374
学科 基础医学
基金 the Science and Technology Research Foundation of Liaoning Provincial Department of Education
文献收藏号 CSCD:3893811

参考文献 共 26 共2页

1.  Lundborg G. Alternatives to autologous nerve grafts. Handchir Mikrochir Plast Chir,2004,36(1):1-7 被引 6    
2.  Weber RV. Bridging the neural gap. Clin Plast Surg,2005,32(4):605-616 被引 4    
3.  Bellamkonda RV. Peripheral nerve regeneration: an opinion on channels, scaffolds and anisotropy. Biomaterials,2006,27(19):3515-3518 被引 19    
4.  Hu I. Repair of extended peripheral nerve lesions in rhesus monkeys using acellular allogenic nerve grafts implanted with autologous mesenchymal stem cells. Exp Neurol,2007,204(2):658-666 被引 1    
5.  Walsh S. Supplementation of acellular nerve grafts with skin derived precursor cells promotes peripheral nerve regeneration. Neuroscience,2009,164(3):1097-1107 被引 15    
6.  Heijke GC. Silicone rubber tubulization in peripheral sensory nerve reconstruction: an experimental study in rabbits. Microsurgery,2001,21(7):306-316 被引 1    
7.  Ikeguchi R. Rat nerve regeneration through a silicone chamber implanted with negative carbon ions. Brain Res Dev Brain Res,2003,140(1):127-131 被引 1    
8.  Sinis N. Neuroma formation in a rat median nerve model: influence of distal stump and muscular coating. Plast Reconstr Surg,2007,119(3):960-966 被引 2    
9.  Chiono V. Chapter 9: Artificial scaffolds for peripheral nerve reconstruction. Int Rev Neurobiol,2009,87(8):173-198 被引 2    
10.  Sun XH. Improving nerve regeneration of acellular nerve allografts seeded with SCs bridging the sciatic nerve defects of rat. Cell Mol Neurobiol,2009,29(3):347-353 被引 14    
11.  Xu YD. Technologies for repairing peripheral nerve injury: Progress in domestic and foreign investigations. Neural Regen Res,2007,2(7):429-432 被引 1    
12.  Sun HZ. Preparation of acellular nerve allografts and analysis of their components. Jiepouxue Jinzhan,2004,10(2):106-108 被引 1    
13.  Haase SC. Recovery of muscle contractile function following nerve gap repair with chemically acellularized peripheral nerve grafts. J Reconstr Microsurg,2003,19(4):241-248 被引 6    
14.  Zhang Y. Tissue engineering peripheral nerve with TGF-beta repair sciatic nerve defect. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi,2007,24(2):394-398 被引 3    
15.  Roddy M. Heterogeneous alterations in human alloimmunity associated with immunization. Transplantation,2005,80(3):297-302 被引 1    
16.  The Ministry of Science and Technology of the People's Republic of China. Guidance Suggestions for the Care and Use of Laboratory Animals,2006 被引 849    
17.  Sondell M. Regeneration of the rat sciatic nerve into allografts made acellular through chemical extraction. Brain Res,1998,795(1/2):44-54 被引 102    
18.  Chen BY. Repair of rat sciatic nerve defect with grafting of rabbit nerve decellulated by chemical extraction. Zhongguo Jiaoxing Waike Zazhi,2003,11(21):1476-1478 被引 1    
19.  Kadoya K. Combined intrinsic and extrinsic neuronal mechanisms facilitate bridging axonal regeneration one year after spinal cord injury. Neuron,2009,29:165-172 被引 15    
20.  Liu CH. A new rapid method of staining thin blood film: first report. J Formos Med Assoc,1953(52):348-352 被引 1    
引证文献 3

1 Ma Xinlong Biomechanical properties of acellular sciatic nerves treated with a modified chemical method Neural Regeneration Research,2011,6(4):252-256
被引 5

2 Ma Xinlong Biomechanical properties of peripheral nerve after acellular treatment Chinese Medical Journal,2011,124(23):3925-3929
被引 0 次

显示所有3篇文献

论文科学数据集
PlumX Metrics
相关文献

 作者相关
 关键词相关
 参考文献相关

版权所有 ©2008 中国科学院文献情报中心 制作维护:中国科学院文献情报中心
地址:北京中关村北四环西路33号 邮政编码:100190 联系电话:(010)82627496 E-mail:cscd@mail.las.ac.cn 京ICP备05002861号-4 | 京公网安备11010802043238号