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文摘
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目的:建立7种龙胆花中龙胆苦苷和獐牙菜苦苷含量测定的HPLC方法.方法:采用微波辅助动态回流法进行提取,色谱条件:Fusion-RP 80 A C_(18)柱(150 mm ×4.6 mm,5 μm);流动相:甲醇-0.2%磷酸溶液梯度洗脱(0～25 min:15%～30%);流速:1 mL/min;柱温:30℃;检测波长240nm.结果:7种龙胆花中獐牙菜苦苷和龙胆苦苷的色谱峰与共存组分完全达到基线分离,线性范围分别为0.105～0.945μg(r=0.999 9),0.3～0.7μg(r=0.999 9),平均加样回收率分别为97.8%(RSD=1.02%),98.9%(RSD=1.51%).结论:所建立的方法测定快速,结果准确可靠. |
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其他语种文摘
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Objective: To establish a HPLC method for the determination of swertiamarin and gentiopico-side in the flowers of seven genfianaceae. Methods: A new method-microwave - assisted reflux with magnetic stir extraction was used. Experiments were performed on Fusion - RP 80 A C_(18) ( 150 mm × 4.6 mm, 5μm) column , with the mobile phase consisting of methanol and 0. 2 % phosphoric acid at a flow rate of 1 mL/min. The operation was conducted in the gradient mode in which the ratio of methanol to 0.2% phosphoric acid varied from 15:85 to 30:70 in 2.5 min. The column temperature was 30 ℃ and the determination wavelength was 240 nm. Results: Chromatographic separation of swertiamarin, gentiop-icroside and the co-existed compounds achieved baseline resolution. The linear range were 0.105～0. 945 μg(r=1.999 9) and 0.3～0.7(r=0.999 9) separately, the average recovery were respectively 97.8% (RSD=1.02%) and 98.9% (RSD = 1.51%). Conclusion: The method is rapid and precise. |
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来源
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中国野生植物资源
,2010,29(2):38-40 【扩展库】
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关键词
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高效液相色谱
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龙胆科植物花
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獐牙菜苦苷
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龙胆苦苷
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地址
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中国科学院西北高原生物研究所, 青海, 西宁, 810008
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1006-9690 |
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学科
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农作物 |
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基金
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国家科技支撑计划项目
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文献收藏号
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CSCD:3859538
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1.
刘圆. RP-HPLC法测定藏药龙胆花的两种原植物白花龙胆和蓝玉簪龙胆中龙胆苦苷.
中草药,2006,37(11):1783
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被引
2
次
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2.
北京医学院.
中草药成分化学,1884:344
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被引
2
次
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3.
GARCIA-AYUSO L E. Determination of the oil content of seeds by focused microwave -assisted soxhlet extraction.
Chromatowaphia,2000,52(1/2):103-108
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被引
1
次
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|
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4.
EIGUREN FERN. Microwave-assisted extraction of organochlo-rine compounds in marine sediments with organized molecular sys-tems.
CHROMATOGRAPHIA,2001,53(7/8):375-379
|
被引
1
次
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5.
PAN Xuejun. Microwave-assisted extraction of tanshinones from salvia mihiorrhiza bunge with analy-sis by high2 performance liquid chromatography.
J Chromatogr:A,2001,922:371-375
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被引
22
次
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6.
万益群. 何首乌中磷脂类化合物的微波辅助萃取/高效液相色谱法测定.
分析测试学报,2008,27(7):782-784
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被引
8
次
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7.
周围. 中药材中拟除虫菊酯类农药残留的微波萃取／气相色谱检测.
分析测试学报,2007,26(6):884-887
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被引
6
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