谷胱甘肽包被的CdSe/CdS量子点的直接水相制备及其对人血淋巴细胞的标记成像
Preparation of GSH Capped CdSe/CdS Core-Shell QDs and Labeling of Human T-Lymphocyte
查看参考文献16篇
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文摘
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首次用谷胱甘肽(GSH)作为稳定剂,在水溶液中制备了稳定地发射绿色荧光和橙色荧光的两种CdSe/CdS核/壳结构的纳米量子点.用紫外-可见分光光度和荧光光谱方法研究了CdSe/CdS量子点的发光特性.透射电镜(TEM)结果表明Cdse/CdS最子点近似球形,在水中分散性良好,比CdSe量子点具有更优异的发光特性,发射光谱和吸收光谱都有红移现象.将CdSe/Gds量子点与鼠抗人CD3抗体连接,制备了水溶件Cdse/CdS-CD3复合物探针,对人血淋巴细胞进行标记和成像.结果表明用该探针对人血淋巴细胞成像清晰,其荧光在30 min的连续蓝光激发下无明显衰退,而FITC荧光在20 min内基本完全猝灭. |
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其他语种文摘
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Two kinds of L-glutathione capped highly fluorescent CdSe/CdS core-shell quantum dots (QDs) emitting green and orange fluorescence at 350 nm excitation were firstly prepared by an aqueous approach and used as fluorescent labels, to link mouse anti-human CD3 which was expressed on human T-lymphocyte. UV-Vis absorption and fluorescence emission spectra of the as-prepared CdSe/CdS core-shell QDs were studied. Compared with the CdSe QDs, a remarkable enhancement in the emission intensity and a red shift of emission wavelength of CdSe/CdS core-shell QDs was observed for the two kinds of QDs emitting green and orange fluorescence. The TEM results showed that the as prepared CdSe and CdSe/CdS QDs dispersed well in aqueous solution, and their shape was approximately spherical, and the CdSe/CdS QDs nano particles emitting green fluorescence are of about 5 nm in diameter. The two kinds of CdSe/CdS QDs were linked with mouse anti-human CD_3 to image human T-lymphocyte. The fluorescent microscopical images of human T-lymphocyte labeled with CdSe/CdS QDs-CD3 and F1TC-CD3 demonstrated that the fluorescent CdSe/CdS QDs exhibited much better photo stability and brighter fluorescence than FITC, showing a good application potential in the immuno-labeling of cells. |
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来源
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光谱学与光谱分析
,2010,30(1):118-122 【核心库】
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DOI
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10.3964/j.issn.1000-0593(2010)01-0118-05
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关键词
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谷胱甘肽
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CdSe/CdS量子点
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免疫荧光标记
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人血淋巴细胞
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地址
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1.
东北大学化学系, 辽宁, 沈阳, 110004
2.
沈阳医学院化学系, 辽宁, 沈阳, 110034
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-0593 |
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学科
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化学 |
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基金
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国家自然科学基金
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辽宁省教育厅项目
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辽宁省自然科学基金
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文献收藏号
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CSCD:3825007
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