外源NO对铜胁迫下番茄幼苗生长及其抗氧化酶编码基因mRNA转录水平的影响
Effects of Exogenous Nitric Oxide on Growth and Transcriptional Expression of Antioxidant Enzyme mRNA in Tomato Seedlings under Copper Stress
查看参考文献18篇
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文摘
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研究了水培条件下外源一氧化氮(NO)对Cu胁迫后番茄幼苗生长及抗氧化酶编码基因mRNA表达的影响.结果表明,0.5 mmol·L~(-1)Cu~(2+)处理使番茄幼苗株高、茎粗、鲜样质量、干样质量明显下降,抑制r幼苗生长,外施0.3 mmol·L~(-1)SNP(NO供体)能够缓解Cu~(2+)对番茄幼苗生长的抑制,显著增加叶片编码过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)基因mRNA的表达量,而0.1 mmol·L~(-1)NAME [N~ω -nitro-L-arginine methyl ester,一氧化氮合成酶(NOS)抑制剂]处理的表达量与Cu胁迫处理相比有所减少,幼苗生长受到更加明显抑制,表明外源NO可以通过提高抗氧化酶的表达水平而缓解Cu胁迫对番茄幼苗的伤害. |
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其他语种文摘
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Heavy metal stress badly affect the crop growth and development, assuaging heavy metal stress to crop show important for crop yield. Here we investigated the effect of SNP (sodium nitropprusside, an exogenous nitric oxide donor) on alleviating copper stress and regulating antioxidant gene transcriptional level in liquid-culture tomato seedlings. Our results showed that copper at 0.5 mmol · L~(-1) significantly suppressed the tomato seedlings growth including plant height, stem thickness, plant fresh weight and plant dry weight, as well as improved the transcriptional levels of antioxidant genes encoding peroxidase (POD), ascorbate peroxi-dase (APX), superoxide dismutase (SOD) and catalase (CAT). Exogenous application of 0.3 mmol · L~(-1) SNP remarkably alleviated copper-inhibition to tomato growth and further enhanced these antioxidant genes transcription, however, additional L-NAME (N~ω-nitro-L-arginine methyl ester),-the special inhibitor of nitric oxide synthase (NOS) obviously aggravated copper-induced inhibitory effect on tomato growth and reduced an-tioxidant enzyme gene transcriptional level. Basing on these results we propose that tomato NOS enzyme-medi-ated NO is necessary for tomato responding to copper stress and NO application lessen copper stress to tomato growth via enhancing antioxidant enzyme gene transcriptional levels. |
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来源
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园艺学报
,2010,37(1):47-52 【核心库】
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关键词
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Cu胁迫
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番茄
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RT-PCR
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地址
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1.
山东农业大学园艺科学与工程学院, 作物生物学国家重点实验室,园艺作物生物学农业部重点开放实验室, 山东, 泰安, 271018
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中国科学院昆明植物研究所, 云南, 昆明, 650204
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0513-353X |
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学科
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园艺 |
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基金
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国家自然科学基金
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山东省农业重大应用技术创新项目
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文献收藏号
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CSCD:3816695
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