芙蓉菊化学成分对体外培养大鼠胰岛分泌胰岛素作用的研究
Effects of chemical constituents of Crossostephium chinense on insulin secretion in rat islets in vitro
查看参考文献17篇
文摘
|
目的:研究芙蓉菊化学成分在体外对培养的大鼠胰岛分泌胰岛素的作用.方法:体外培养大鼠胰岛,通过受试化合物作用前后胰岛分泌胰岛素水平的比较,考察芙蓉菊化学成分艾菊素、万寿菊黄素-3,6,7-三甲醚、5-O-甲基-myo-肌醇、东莨菪素和东莨菪苷对胰岛分泌胰岛素的作用,并与阳性对照药格列苯脲进行比较.结果:万寿菊黄素-3,6,7-三甲醚对胰岛分泌胰岛素的促进作用约为空白对照组分泌水平的2倍.5-O-甲基-myo-肌醇对胰岛分泌胰岛素亦有促进作用,约为空白对照组分泌水平的1.5倍.但是,东莨菪素对胰岛分泌胰岛素有抑制作用,约比空白对照低1.9倍.结论:对体外培养的大鼠胰岛分泌胰岛素功能,艾菊素、万寿菊黄素-3,6,7-三甲醚和5-O-甲基-myo-肌醇有促进作用;东莨菪素有抑制作用. |
其他语种文摘
|
Objective:To investigate the effects of the chemical constituents of the whole herbs of Crossostephium chinense on insulin secretion in rat islets.Method:Islets were isolated from rat pancreata.cultured in vitro,and measured by color signals of dithizone stained digestion solution for detection of pancreatic islets.The morphological observation of islets was carried out by inverted microscope.The effects of test compounds,scopoletin(1),scopolin(2),tanacetin(3),quercetagetin-3,6,7-trimethylether(4)and 5-O-methyl-myo-inositol(5)isolated from the whole herbs of C.chinense,on the insulin secreting level from islets were compared with those of glybenclamide as a positive control substances,and the difference in insulin secreting level from islets between the presence and absence of test compounds was assayed.Result:There was no difference in basal insulin secretion before and after 2 h incubation period of rat islets.The islets treated with quercetagetin-3,6,7-trimethylether have about 2-fold higher insulin secreting level (P<0.01)compared a normal control group.The islets treated with 5-O-methyl-myo-inositol have about 1.5-fold higher insulin secreting level(P<0.05)compared to a normal control group.Whereas the islets treated with scopoletin show about 1.9-fold lower basal insulin secreting level(P<0.05)than a normal control group.Conclusion:In this paper the developed cultivation method of isolated pancreatic islets from rat can be used as a kind of islet-based drug screening model for diabetes mellitus in vitro.Quercetagetin-3,6,7-trimethylether and 5-O-methyl-myo-inositol could enhance rat islet insulin secretion and further in vivo studies are needed to clarify the nature of such an observation.However,scopletin suppress rat islet insulin secretion. |
来源
|
中国中药杂志
,2009,34(11):1401-1405 【核心库】
|
关键词
|
芙蓉菊
;
万寿菊黄素-3,6,7-三甲醚
;
5-O-甲基-myo-肌醇
;
大鼠胰岛
;
胰岛素
|
地址
|
1.
北京大学药学院天然药物学系, 天然药物及仿生药物国家重点实验室, 北京, 100191
2.
中国科学院过程工程研究所, 生化工程国家重点实验室, 北京, 100080
|
语种
|
中文 |
文献类型
|
研究性论文 |
ISSN
|
1001-5302 |
学科
|
中国医学 |
基金
|
国家973计划
|
文献收藏号
|
CSCD:3778974
|
参考文献 共
17
共1页
|
1.
Wild S. Global prevalence of diabetes.
DIABETES CARE,2004,27:1047
|
被引
339
次
|
|
|
|
2.
侯宽昭.
广州植物志,1956:537
|
被引
2
次
|
|
|
|
3.
黄泰康.
现代本草纲目.下册,2001:1981
|
被引
1
次
|
|
|
|
4.
车今智. 芙蓉菊寡糖的分离纯化及其生物活性的研究.
天然产物研究与开发,2004,16(5):458
|
被引
5
次
|
|
|
|
5.
傅德贤. 芙蓉菊中黄酮类化学成分的研究.
天然产物研究与开发,2008,20(2):265
|
被引
4
次
|
|
|
|
6.
杨秀伟. 芙蓉菊化学成分研究.
中国中药杂志,2008,33(8):905
|
被引
9
次
|
|
|
|
7.
杨秀伟. 芙蓉菊中艾菊素和草蒿素结构的NMR信号表征.
波谱学杂志,2008,25(1):117
|
被引
4
次
|
|
|
|
8.
Wu Qi. Novel sesquiterpene and coumarin constituents from the whole herbs of Crossostephium chinense.
Journal of Asian Natural Products Research,2009,11(1):85
|
被引
7
次
|
|
|
|
9.
Lebrun P. Potent diazoxide analogue activating ATP-sensitive K~+ channels and inhibiting insulin release.
DIABETOLOGIA,2000,43:723
|
被引
1
次
|
|
|
|
10.
陶义训.
免疫学和免疫学检验,1997:156
|
被引
2
次
|
|
|
|
11.
Efanova I B. Glucose and tolbutamide induce apoptosis in pancreatic β-cells.
Journal of Biological Chemistry,1998,273(50):33501
|
被引
4
次
|
|
|
|
12.
张卓然.
实用细胞培养技术,1999:66
|
被引
1
次
|
|
|
|
13.
Sun A M. Insulin secretion from a continuously subcultivable isolate derived from foetal bovine pancreata beta cells:I.Monolayer cultures.
Biochemical and Biophysical Research Communications,1977,79(1):185
|
被引
1
次
|
|
|
|
14.
Ono J. Long-term culture of pancreatic islet cells with special reference to the β-cell function.
In Vitro,1979,15:95
|
被引
1
次
|
|
|
|
15.
Lambert A E. Monolayer cell culture of neonatal rat pancreas:Light microscopy and evidence for immunoreactive insulin synthesis and release.
Endocrinol,1972,90:239
|
被引
5
次
|
|
|
|
16.
梁敬钰.
一种防治糖尿病的天然化合物及其制备方法与药物用途.PCT:WO 2004/039759 A1,2004
|
被引
1
次
|
|
|
|
17.
纪莎. 口服降糖药的分类和特点.
福建医药杂志,2001,23(6):119
|
被引
2
次
|
|
|
|
|