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超大孔聚苯乙烯微球固定蛋白质
Separation and Immobilization of Protein on Macroporous Polystyrene Chromatography Microspheres

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曲瑞芳 1   孔英俊 2   曲建波 2   马光辉 2   马润宇 1   苏志国 2   罗坚 2 *  
文摘 采用琼脂糖对孔径为300~500 nm的超大孔聚苯乙烯(MPS)微球进行亲水化修饰,并在修饰后的表面上偶联病毒蛋白颗粒(RV),用于蛋白质的分离纯化实验.结果表明,经过修饰的MPS层析介质具有优异的流体力学性能,层析过程的最高流速可达1120 cm/h,对病毒蛋白颗粒的固定量为0.638 mg/g,是商品化介质Sepharose 4FF的2倍.采用RV-MPS介质对病毒蛋白颗粒抗体进行了分离纯化,收率为29.22%,高于Sepharose 4FF的18.92%.
其他语种文摘 Macroporous polystyrene microspheres with pore diameters between 300~500 nm were modified with hydrophilic agarose, and the hydrophilic surface was then immobilized with virus protein particles for protein purification experiments. The results showed that the modified macroporous polystyrene microspheres had excellent hydrodynamic characteristics, with a working flow rate as high as 1120 cm/h. The protein separation time was also greatly reduced compared with a commercially available agarose medium. The virus protein particle coupling capacity of 0.638 mg/g was about two times of commercially agarose medium. A recovery rate of 29.22% was achieved using RV-MPS medium, higher than 18.92% using commercially agarose medium.
来源 过程工程学报 ,2009,9(6):1164-1168 【核心库】
关键词 超大孔聚苯乙烯微球 ; 流体力学特性 ; 蛋白质 ; 分离 ; 固定
地址

1. 北京化工大学生命科学与技术学院, 北京, 100029  

2. 中国科学院过程工程研究所, 生化工程国家重点实验室, 北京, 100190

语种 中文
文献类型 研究性论文
ISSN 1009-606X
学科 化学;微生物学
基金 国家自然科学基金 ;  国家863计划
文献收藏号 CSCD:3763596

参考文献 共 11 共1页

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引证文献 2

1 李燕 超大孔聚(苯乙烯-甲基丙烯酸缩水甘油酯)共聚微球的制备及孔结构的调控 过程工程学报,2010,10(3):608-612
被引 1

2 康丽梅 超大孔离子交换制备色谱分离纯化高活性凝血因子Ⅷ 色谱,2012,30(6):618-623
被引 1

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