Pluronic嵌段共聚物磁性纳米颗粒的制备及其对漆酶的分离纯化
Preparation of Pluronic-based Magnetic Nanoparticles and Their Application in Purification of Laccase
查看参考文献29篇
文摘
|
选择合适的双亲性嵌段共聚物Pluronic产品接枝到Fe3O4粒子的表面,合成了粒径均一、亲水性好、非特异性吸附低的纳米级磁性分离载体(PMNPs).通过对漆酶发酵液的分离纯化研究了Pluromic对排除蛋白非特异性吸附的作用.结果表明,PMNPs对漆酶的最大吸附量可达O.535 mg/mg,在5-40℃内基本不受温度变化影响,对漆酶发酵液的分离纯化因子为3.4,酶活收率达62.9%,可实现一步分离提纯. |
来源
|
过程工程学报
,2009,9(3):456-461 【核心库】
|
关键词
|
PEO-PPO-PEO
;
磁纳米颗粒
;
吸附
;
分离纯化
;
漆酶
|
地址
|
1.
中国科学院过程工程研究所, 绿色过程与工程重点实验室, 北京, 100190
2.
中国科学院过程工程研究所, 生化工程国家重点实验室, 北京, 100190
|
语种
|
中文 |
文献类型
|
研究性论文 |
ISSN
|
1009-606X |
学科
|
生物工程学(生物技术) |
基金
|
国家自然科学基金
;
国家863计划
|
文献收藏号
|
CSCD:3763544
|
参考文献 共
29
共2页
|
1.
Abudiab T. Preparation of Magnetic Immobilized Metal Affinity Separation Media and Its Use in the Isolation of Proteins.
Journal of Chromatography A,1998,795(2):211-217
|
被引
17
次
|
|
|
|
2.
Prikryl P. Magnetic IDA-modified Hydrophilic Methacrylate-based Polymer Microspheres for IMAC Protein Separation.
J.Sep.Sci,2006,29:2541-2549
|
被引
2
次
|
|
|
|
3.
Berry C C. Dextran and Albumin Derivatised Iron Oxide Nanoparticles:Influence On Fibroblasts in vitro.
Biomaterials,2003,24:4551-4557
|
被引
24
次
|
|
|
|
4.
Chatterjee J. Synthesis and Characterization of Heat-stabilized Albumin Magnetic Microspheres.
Colloid and Polymer Science,2001,279:1073-1081
|
被引
2
次
|
|
|
|
5.
Paul K G. Synthesis of Ultrasmall Superparamagnetie Iron Oxides Using Reduced Polysaccharides.
Bioconjugate Chemistry,2004,15:394-401
|
被引
5
次
|
|
|
|
6.
Mornet S. A Method for Synthesis and Functionalization of Ultrasmall Superparamagnetic Covalent Carriers Based on Maghemite and Dextran.
Journal of Magnetism and Magnetic Materials,2005,293:127-134
|
被引
3
次
|
|
|
|
7.
Hassan E E. Optimized Formulation of Magnetic Chitosan Microspheres Containing the Anticancer Agent,Oxantrazole.
Pharmaceutical Research,1992,9:390-397
|
被引
35
次
|
|
|
|
8.
Suzuki M. preparation and Characteristics of Magnetite-labelled Antibody with the Use of Poly(ethylene glycol)Derivatives.
Biotechanol.Appl.Biochem,1995,21:335-345
|
被引
2
次
|
|
|
|
9.
Chastellain M. Particle Size Investigations of a Multistep Synthesis of PVA Coated Superparamagnetic Nanoparlicles.
Journal of Colloid and Interface Science,2004,278:353-360
|
被引
8
次
|
|
|
|
10.
马志亚.
超顺磁性聚合物纳米载体的合成、表征及生物亲和分离研究,2000:25-136
|
被引
1
次
|
|
|
|
11.
Harris L A. Magnetite Nanoparticle Dispersions Stabilized with Triblock Copolymers.
Chemistry of Materials,2003,15:1367-1377
|
被引
21
次
|
|
|
|
12.
Baldrian P. Fongal Laccases-Occurrence and Properties.
Fems Microbiology Reviews,2006,30(2):215-242
|
被引
120
次
|
|
|
|
13.
Yaropolov A I. Laccase:Properties,Catalytic Mechanism,and Applicability.
Applied Biochemistry and Biotechnology,1994,49:257-80
|
被引
29
次
|
|
|
|
14.
Solomon E I. Multicopper Oxidases and Oxygenases.
Chemical Reviews,1996,96:563-605
|
被引
1
次
|
|
|
|
15.
Rodriguez Couto S. Industrial and Biotechnological Applications of Laccases:A Review.
Biotechnology Advances,2006,24:500-513
|
被引
57
次
|
|
|
|
16.
Litthauer D. Purification and Kinetics of a Thermostable Laccase from Pycnoporus sanguineus (SCC 108).
Enzyme and Microbial Technology,2007,40(4):563-568
|
被引
8
次
|
|
|
|
17.
Pich A. Composite Magnetic Particles as Carriers for Laccase from Trametes Versicolor.
Macromolecular Bioscience,2006,6(4):301-310
|
被引
2
次
|
|
|
|
18.
Alexandridis P. Micellization of Poly(ethylene oxide)-Poly(propylene oxide)-Poly(ethylene oxide) Tfiblock Copolymers in Aqueous Solution Thermedymnics of Copolymer Association.
Macromolecules,1994,27(9):2414-2425
|
被引
35
次
|
|
|
|
19.
Chen S. Temperature-responsive Magnetite/PEO-PPO-PEO Block Copolymer Nanoparticles for Conuolled Drug Targeting Delivery.
Langmnir,2007,23(25):12669-12676
|
被引
5
次
|
|
|
|
20.
Nguyen H K. Evaluation of Polyether-Polyethyleneimine Graft Copolymers as Gene Transfer Agents.
Gene Thor,2000,7:126-138
|
被引
1
次
|
|
|
|
|