乳突果苗的组织培养及脂氧合酶的诱导
Tissue culture of Adelostemma gracillimum seedlings and the inducement of lipoxygenase
查看参考文献13篇
文摘
|
脂氧合酶(LOX)是植物十八碳酸途径中一个很重要的酶,该酶作用的产物在植物生长发育过程以及在植物对环境胁迫反应中起着重要作用.首次建立了萝藦科植物乳突果苗的培养体系,并用仿真菌环境(几丁质)对乳突果组培苗进行刺激诱导,通过LC-ESI-MS检测脂氧合酶反应的产物.粗酶活性鉴定结果显示,经150 mg/L几丁质诱导9 h的乳突果组培苗产生了9-LOX,该酶可催化亚油酸生成9,10,11-三羟基-12-十八碳烯酸.推测在乳突果组培苗中,几丁质诱导的十八碳酸代谢途径沿着9-LOX方向进行. |
其他语种文摘
|
Lipoxygenases are important enzymes in plant octadecanoic acid pathway. Products of this enzyme have important functions in plant growth and development,as well as in response to environmental stress. Culture system of Adelostemma gracillimum was the first time to be constructed in Asclepiadaceae. Chitosan was used in the inducement experiment on A. gracillimum seedlings and the products of LOX were detected through LOESI-MS. The results showed that A. gracillimum seedlings induced by 150 mg/L chitosan for 9 h produced 9-LOX and the enzyme can catalyze linoleic acid to 9,10,U-trihydroxy-12-octadecenoic acid. It was speculated that octadecanoic acid pathway induced by chitosan differentiated to 9-LOX orientation. |
来源
|
广西植物
,2009,29(3):386-389 【核心库】
|
关键词
|
乳突果
;
脂氧合酶
;
十八碳酸途径
;
几丁质
|
地址
|
1.
西南林学院, 云南, 昆明, 650224
2.
中国科学院昆明植物研究所, 云南, 昆明, 650204
|
语种
|
中文 |
文献类型
|
研究性论文 |
ISSN
|
1000-3142 |
学科
|
植物学 |
基金
|
云南省教育厅科研基金
|
文献收藏号
|
CSCD:3762618
|
参考文献 共
13
共1页
|
1.
赵沛基. 乳突果组织培养及愈伤组织中的多羟基脂肪酸.
中国植物学会七十周年年会论文摘要汇编,2003:441-442
|
被引
3
次
|
|
|
|
2.
Brash AR. Lipoxygenases:occurrence,functions,catalysis,and acquisition of substrate.
Journal of Biological Chemistry,1999,274:23679-23682
|
被引
45
次
|
|
|
|
3.
Creelman RA. Biosynthesis and action of jasmonates in plants.
Annual Review of Plant Physiology and Plant Molecular Biology,1997,48:355-381
|
被引
161
次
|
|
|
|
4.
Gbel C. Oxylipin profiling in pathogen-infected potato leaves.
Biochimica and Biophysica Acta,2002,1 584:55-64
|
被引
1
次
|
|
|
|
5.
Grechkin A. Recent developments in biochemistry of the plant lipoxygenase pathway.
Progress in Lipid Research,1998,37(5):317-352
|
被引
26
次
|
|
|
|
6.
黄建昌. 模拟酸雨对番木瓜不同成熟度叶片膜脂过氧化作用的影响.
广西植物,2005,25(6):562-565
|
被引
1
次
|
|
|
|
7.
黄涛. 影响沙田柚叶片离体培养的因素研究.
广西植物,2003,23(6):561-564
|
被引
5
次
|
|
|
|
8.
Kolomets MV. A leaf lipoxygenase of potato induced specifically by pathogen infection.
Plant Physiology,2000,124:1121-1130
|
被引
3
次
|
|
|
|
9.
Lee A. Inverse correlation between jasmonic acid and salicylic acid during early wound response in rice.
Biochemical and Biophysical Research Communications,2004,318(3):734-738
|
被引
9
次
|
|
|
|
10.
李靖. 水杨酸诱导美登木悬浮细胞产生脂氧合酶及多羟基脂肪酸的研究.
云南植物研究,2004,26(5):543-548
|
被引
5
次
|
|
|
|
11.
Santino A. Cloning and characterisation of an almond 9-lipoxygenase expressed early during seed development.
Plant Science,2005,168(3):699-706
|
被引
3
次
|
|
|
|
12.
Sharma R. Fruit calcium content and lipoxygenase activity in relation to albinism disorder in strawberry.
Sci Hort,2006,107:150-154
|
被引
1
次
|
|
|
|
13.
Terp N. Lipoxygenases during Brassica napus seed germination.
PHYTOCHEMISTRY,2006,67(18):2030-2040
|
被引
4
次
|
|
|
|
|