打顶对烤烟植株钾素代谢和钾离子通道基因表达的影响
Effects of Topping on Potassium Metabolism and Expression of Potassium Channels in Tobacco Plants
查看参考文献38篇
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文摘
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[目的]定量检测打顶对烟株钾离子通道基因表达的影响,为从分子水平上研究打顶对烟草钾素营养调控机理提供理论依据.[方法]在盆栽基质培养条件下,利用实时荧光定量PCR技术检测了打顶后(1 h、5 h,24 h和14 d)烟株钾离子通道基因表达的变化,并通过酶联免疫法分析烟株内源激素的含量.[结果]与不打顶处理比较,打顶能够降低整株钾累积量,有利于钾素向叶和根中分配;能够抑制NKT1和NTORK1基因在叶中表达,诱导其在根中表达.NKT1叶中表达量高于根,NTORK1在打顶后24 h内叶中表达量高于根,打顶后14 d根中表达量高于叶.根中钾离子通道基因表达是调控根系钾素累积和决定地上部K+浓度的关键.打顶有利于K+从根系细胞质膜中流出,导致由根系向地上部运输的K+减少,从而使烟叶中钾累积量降低.打顶对钾离子通道基因表达的调控作用与烟株内源激素水平变化有关,打顶后叶中IAA含量降低,根中IAA含量增加,其中14d时根中IAA比不打顶处理增加了140.7%.[结论]打顶后烟株内源激素水平变化可能是调控钾离子通道基因表达的主要原因,而钾离子通道基因表达的变化直接影响着烟株体内钾素累积和分配. |
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来源
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中国农业科学
,2009,42(3):854-861 【核心库】
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关键词
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打顶
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烤烟
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钾离子通道
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钾素
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地址
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1.
中国科学院亚热带农业生态研究所, 中国科学院亚热带农业生态过程重点实验室, 湖南, 长沙, 410125
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中国科学院亚热带农业生态研究所, 中国科学院亚热带农业生态重点实验室, 湖南, 长沙, 410125
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贵州省铜仁地区烟草公司, 贵州, 铜仁, 554300
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华中农业大学资源与环境学院, 湖北, 武汉, 430070
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0578-1752 |
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学科
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农作物 |
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基金
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中国科学院知识创新工程重要方向项目
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贵州省烟草专卖公司科技计划项目
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文献收藏号
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CSCD:3738755
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