滤食性鲢、鳙肠含物PCR-DGGE指纹分析
PCR-DGGE fingerprinting analysis on intestinal contents of filter-feeding fishes (Hypophthalmichthys molirix and Aristichthys nobilis)
查看参考文献30篇
|
文摘
|
以采自武汉东湖的滤食性鲢、鳙为对象,通过PCR-DGGE并结合序列分析对其肠道微生物及肠含物中残留的食物组分进行了探索研究.在所有个体中都能检测到不同的PCR-DGGE指纹谱带,其中包括肠道细菌在内的原核谱带较多,真核谱带相对较少;分析结果表明针对鲢、鳙肠含物这一特殊生境样品进行PCR-DGGE指纹分析是可行的.PCR-DOGE指纹结构及针对部分特定PCR-DGGE谱带的序列分析显示,从武汉东湖采集的鲢、鳙在食性上存在很大的重叠,并没有像基于常规食性分析文献报道的那样明显不同.基于肠含物DNA来进行鱼类食性分析的方法不受对食物碎片分类鉴定的制约,可同时对多个样品平行进行分析,且不同研究者间的研究结果便于进行比较,以便总结生态学的内在规律. |
|
其他语种文摘
|
PCR-DGGE fingerprinting was explored in the present study to analyze intestinal contents of filter-feeding fishes (Hypophthalmichthys molirix and Aristichthys nobilis) , which were sampled from the Donghu Lake, Wuhan. Different microbial bands (16S rDNA) and eukaryotic bands (18S rDNA) were detected in all the individuals, and the numbers of 16S rDNA bands were more than that of 18S rDNA bands. Results of the present study suggested that it is possible and useful to apply PCR-DGGE fingerprinting to studying the intestinal contents of filter-feeding fishes. Additionally, PCR-DGGE fingerprinting and sequences analysis of the DGGE bands indicated that intestinal contents composition of silver carp and bighead carp, which had been found to be significantly different in traditional dietary analyses with microscopy identification, was not significantly different. These results suggested that a significant portion of planktonic organisms were fed by the silver carp and bighead carp. In DNA based dietary analyses, multiple samples can be studied simultaneously with a standardized set of procedures, making it practical to perform comparative analyses, and furthermore, this comparison makes it possible to elucidate some objective ecological rules. |
|
来源
|
水产学报
,2009,33(6):972-979 【核心库】
|
|
关键词
|
鲢
;
鳙
;
PCR-变性梯度凝胶电泳
;
肠含物
;
武汉东湖
|
|
地址
|
中国科学院水生生物研究所, 中国科学院水生生物多样性与保护重点实验室, 湖北, 武汉, 430072
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1000-0615 |
|
学科
|
水产、渔业 |
|
基金
|
国家973计划
;
国家自然科学基金
;
中国科学院水生生物研究所淡水生态与生物技术国家重点实验室基金
|
|
文献收藏号
|
CSCD:3727681
|
参考文献 共
30
共2页
|
|
1.
钟麟.
家鱼的生物学和人工繁殖,1965
|
被引
11
次
|
|
|
|
|
2.
倪达书. 花鲢和白鲢的食料问题.
动物学报,1954,6(1):59-71
|
被引
18
次
|
|
|
|
|
3.
陈少莲. 东湖放养鲢、鳙鱼种的食性分析.
水库渔业,1982(3):21-26
|
被引
12
次
|
|
|
|
|
4.
Yan Q Y. Genetic diversity of plankton community as depicted by PCR-DGGE fingerprinting and its relation to morphological composition and environmental factors in Lake Donghu.
Microbial Ecology,2007,54(2):290-297
|
被引
29
次
|
|
|
|
|
5.
Yan Q Y. Plankton community succession in artificial systems subjected to cyanobacterial blooms removal using chitosanmodified soils.
Microbial Ecology,2009,58(1):47-55
|
被引
6
次
|
|
|
|
|
6.
Yu Y H. Spatiotemporal heterogeneity of plankton communities in Lake Donghu,China,as revealed by PCR-denaturing gradient gel electrophoresis and its relation to biotic and abiotic factors.
FEMS Microbiology Ecology,2008,63(3):328-337
|
被引
16
次
|
|
|
|
|
7.
余育和. DNA指纹分析技术在群落级生命系统应用的可能性.
水生生物学报,2004,28(5):457-463
|
被引
22
次
|
|
|
|
|
8.
颜庆云. 洞庭湖浮游生物群落DNA指纹拓扑结构与物种组成对应关系.
生态学报,2005,25(9):2221-2226
|
被引
21
次
|
|
|
|
|
9.
Thompson J D. The CLUSTAL_X windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools.
Nucleic Acids Research,1997,25(24):4876-4882
|
被引
2393
次
|
|
|
|
|
10.
FAO.
Fishery statistics,catches and landings,1991
|
被引
1
次
|
|
|
|
|
11.
农业部渔业局.
中国渔业统计年鉴,2005
|
被引
1
次
|
|
|
|
|
12.
陈少莲. 鲢、鳙在东湖生态系统的氮、磷循环中的作用.
水生生物学报,1991,15:9-26
|
被引
3
次
|
|
|
|
|
13.
杨宇峰. 武汉东湖主要养殖水域的浮游甲壳动物群落分析.
生态学报,2002,22:318-325
|
被引
7
次
|
|
|
|
|
14.
Andersson G. Effects of planktivorous and benthivorous fish on organisms and water chemistry in eutrophic lakes.
HYDROBIOLOGIA,1978,59:9-15
|
被引
2
次
|
|
|
|
|
15.
Arcifa M S. Fishzooplankton interactions and their effects on water quality of a tropical Brazilian reservoir.
HYDROBIOLOGIA,1986,139:49-58
|
被引
1
次
|
|
|
|
|
16.
Xie P. Practical success of biomanipulation using filter-feeding fish to control cyanobacteria blooms:a synthesis of decades of research and application in a subtropical hypereutrophic lake.
The Scientific World Journal,2001,1:337-356
|
被引
63
次
|
|
|
|
|
17.
刘恩生. 太湖鲢、鳙的食物组成及渔获量变化原因分析.
水利渔业,2007,27:72-74
|
被引
11
次
|
|
|
|
|
18.
刘恩生. 太湖鲚鱼和鲢、鳙鱼的食物组成及相互影响分析.
湖泊科学,2007,19:451-456
|
被引
12
次
|
|
|
|
|
19.
Symondson W O C. Molecular identification of prey in predator diets.
Molecular Ecology,2002,11:627-641
|
被引
37
次
|
|
|
|
|
20.
Sheppard S K. Advances in molecular ecology:tracking trophic links through predator-prey food-webs.
Functional Ecology,2005,19:751-762
|
被引
24
次
|
|
|
|
|
了解气象卫星更多信息，请访问