评价γ干扰素释放分析T-SPOT.TB在肺外结核病诊断中的敏感性
Sensitivity of Interferon-gamma Release Assay T-SPOT. TB in Diagnosing Extrapulmonary Tuberculosis
查看参考文献9篇
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文摘
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目的 评价γ干扰素释放分析T-SPOT.TB检测在肺外结核病诊断中的敏感性.方法 对北京协和医院根据细菌学或/和组织学诊断的肺外结核患者31例,应用酶联免疫斑点技术检测6 kD早期分泌靶向抗原和10 kD培养滤过蛋白肽段库刺激后外周血中释放γ-干扰素的特异性T细胞.结果 31例肺外结核患者中29例T-SPOT.TB检测阳性,敏感性为93.5%(95%CI 84.8%～100%),6 kD早期分泌靶向抗原肽段库刺激后斑点形成细胞数中位数为196/10~6 PB-MCs(4分位间距72～532/10~6 PBMCs),10 kD培养滤过蛋白肽段库刺激后斑点形成细胞数中位数为276/10~6 PBMCs(4分位间距72～568/10~6 PBMCs),对两个肽段库总的斑点形成细胞中位数为612/10~6 PBMCs(4分位间距192～1152/10~6PBMCs).结论 T-SPOT.TB检测对肺外结核诊断具有较高的敏感性. |
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其他语种文摘
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Objective To evaluate the sensitivity of an interferon-gamma release assay T-SPOT.TB in the diagnosis of bacteriologically or histologically confirmed extrapulmonary tuberculosis. Method Totally 31 patients with bacteriologically or histologically confirmed extrapulmonary tuberculosis in Peking Union Medical College Hospital received T-SPOT.TB assay to detect early secreting antigen target 6 or culture filtrate protein 10 peptides-specific T cells in the peripheral blood mononuclear cells(PBMCs). Results T-SPOT.TB assay showed positive results in 29 patients with extrapulmonary tuberculosis and the sensitivity was 93.5%(95% CI 84.8%-100%). The median of spot forming cells(SFCs) in response to early secreting antigen target 6 peptides was 196/10~6PBMCs (interquartile range,72-532/10~6 PBMCs),the median of SFCs in response to culture filtrate protein 10 peptides was 276 SFCs/10~6 PBMCs (interquartile range,72-568/10~6 PBMCs),and the median of the incorporate SFCs was 612/10~6 PBMCs (interquartile range,192-1 152/10~6 PBMCs). Conclusion T-SPOT.TB is highly sensitive in diagnosing extrapulmonary tuberculosis. |
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来源
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中国医学科学院学报
,2009,31(4):449-452 【核心库】
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DOI
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10.3881/j.issn.1000-503x.2009.04.013
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关键词
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肺外结核
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敏感性
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T-SPOT.TB
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6kD早期分泌靶向抗原肽段库
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10kD培养滤过蛋白肽段库
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γ干扰素释放分析
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地址
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中国医学科学院,北京协和医学院,北京协和医院感染科, 北京, 100730
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-503X |
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学科
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内科学 |
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文献收藏号
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CSCD:3712264
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参考文献 共
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