基因工程大肠杆菌合成γ-聚谷氨酸
Biosynthesis of Poly-γ-glutamate Acid by Escherichia coli
查看参考文献10篇
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文摘
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利用PCR方法,从自身不合成γ-聚谷氨酸(γ-PGA)的Bacillus subtilis 168菌的基因组DNA中扩增γ-PGA合成基因ywsC,ywtA和ywtB,测序并对该基因编码区进行序列分析,比对结果表明,扩增的ywsC,ywtA和ywtB与文献报道的相似度为100%.将3个基因连接到pTrcHisA载体后转化至E coli TOP10及E coli BL21(DE3)宿主菌表达,结果宿主菌细胞均具备了γ-PGA生物合成能力,产量最高达到0.134 g/L. |
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其他语种文摘
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The genes,ywsC,ywtA and ywtB,involving in biosynthesis of poly-γ-glutamic acid were amplified by PCR from the genome of Bacillus subtills 168.Sequence blast result showed that the sequence of target genes was 100% the same as that of B.subtilis 168.The genes were then inserted to the plasmid pTrcHisA and transformed into E.coli TOP10 and E.coli BL21(DE3) host cells.The result indicated that both the strains obtained the ability to biosynthesize poly-γ-glutamic acid,and the highest yield of γ-PGA reached 0.134 g/L. |
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来源
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过程工程学报
,2009,9(4):791-795 【核心库】
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关键词
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γ-聚谷氨酸
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枯草芽孢杆菌B.subtilis 168
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大肠杆菌TOP10
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大肠杆菌BL21(DE3)
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地址
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中国科学院过程工程研究所, 生化工程国家重点实验室, 北京, 100190
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1009-606X |
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学科
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分子生物学 |
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基金
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国家自然科学基金
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国家973计划
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文献收藏号
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CSCD:3659328
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