Interleukin-6, desmosome and tight junction protein expression levels in reflux esophagitis-affected mucosa
查看参考文献32篇
文摘
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AIM: To investigate the correlation between the expression levels of interleukin (IL)-6 and proteins in tight junctions (TJs) in the esophageal mucosa of rats modeling different types of reflux esophagitis (RE), and the ability of aluminum phosphate to protect against RE-induced mucosal damage via these proteins. METHODS: Male SPF Wistar rats aged 56 d were divided randomly into acid RE, alkaline RE, mixed RE, and control groups. Various surgical procedures were performed to establish rat models of acid RE. At 14 d after the procedure, some of the rats started aluminum phosphate treatment. Transmission electron microscopy (TEM) was used to observe the morphological features of TJs and desmosomes in the esophageal epithelium. Immunohistochemical methods and Western blotting were used to measure expression of claudin 1, occludin, ZO-1, JAM-1, DSG-1 and IL-6; reverse transcription polymerase chain reaction (RTPCR) was used to measure expression of mRNA of claudin 1, occludin, ZO-1, JAM-1, DSG-1 and IL-6. RESULTS: At day 14 after the procedures, an RE model was established in all subsequently sacrificed rats of groups A, B and C. By both gross and microscopic observation, the mucosa was damaged and thickened as the disease progressed. With TEM observation, a widened intercellular space was noticed, with significantly fewer desmosomes. Immunohistochemistry showed significantly higher levels of all proteins in all RE models compared to control rats at 3 d after operation (65.5% ± 25.6% vs 20.5% ± 2.1%, P < 0.05, respectively). At 14 d after operation, along with continuing hyperplasia in the basal layer, the expression of TJ proteins in individual cells gradually decreased (12.4% ± 2.1% vs 20.5% ± 2.1%, P < 0.05, respectively). Western blottings and RT-PCR showed a directly proportional increase in IL-6 levels in relation to TJ proteins, as compared to controls (0.878 ± 0.024 vs 0.205 ± 0.021 and 0.898 ± 0.022 vs 0.205 ± 0.021, P < 0.05, respectively). Upon treatment with aluminum phosphate, however, these protein levels were restored to normal gradually over 30-60 d in rats with acid RE (30.4% ± 2.1% vs 20.5% ± 2.1%, P > 0.05, treated vs untreated, respectively). These levels increased in the rat with alkaline RE, and this increase was accompanied by continued hyperplasia in comparison with controls (85.5% ± 25.6% vs 20.5% ± 2.1%, P < 0.05, respectively). Furthermore, the expression of TJ proteins was not correlated significantly with that of IL-6 in this group. CONCLUSION: These findings indicate that TJ proteins are highly expressed as an early molecular event involved in RE development, and that IL-6 is an inflammatory factor in this process. |
来源
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World Journal of Gastroenterology
,2009,15(29):3621-3630 【核心库】
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关键词
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Reflux esophagitis
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Desmosome
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Tight junction
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Proteins
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Mucosa
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地址
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1.
Department of Digestive Diseases, Central Hospital of Daqing Youtian,China, Heilongjiang, Daqing, 163000
2.
Department of Digestive Diseases, Shengjing Hospital of China Medical University, Liaoning, Shenyang, 110-744
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语种
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英文 |
文献类型
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研究性论文 |
ISSN
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1007-9327 |
学科
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内科学 |
基金
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A grant from the Doctoral Program of China Medical University
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文献收藏号
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CSCD:3614626
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参考文献 共
32
共2页
|
1.
Langbein L. Tight junctions and compositionally related junctional structures in mammalian stratified epithelia and cell cultures derived therefrom.
European Journal of Cell Biology,2002,81:419-435
|
被引
2
次
|
|
|
|
2.
Calabrese C. Dilated intercellular spaces as a marker of oesophageal damage:comparative results in gastro-oesophageal reflux disease with or without bile reflux.
Alimentary Pharmacology and Therapeutics,2003,18:525-532
|
被引
10
次
|
|
|
|
3.
Asaoka D. Altered localization and expression of tight-junction proteins in a rat model with chronic acid reflux esophagitis.
Journal of Gastroenterology,2005,40:781-790
|
被引
4
次
|
|
|
|
4.
Miwa H. GERD and tight junction proteins of the esophageal mucosa.
Nippon Rinsho/Japanese Journal of Clinical Medicine,2004,62:1441-1446
|
被引
1
次
|
|
|
|
5.
Yu Q. Improved technique for the rat model of acid reflux esophagitis.
Zhongguo Zhongxiyi Jiehe Xiaohua Zazhi,2002,10:74-75,78
|
被引
2
次
|
|
|
|
6.
Chen X. An esophagogastroduodenal anastomosis model for esophageal adenocarcinogenesis in rats and enhancement by iron overload.
Carcinogenesis,1999,20:1801-1808
|
被引
6
次
|
|
|
|
7.
Ireland AP. Gastric juice protects against the development of esophageal adenocarcinoma in the rat.
Annals of Surgery,1996,224:358-370,370-371
|
被引
3
次
|
|
|
|
8.
Wang W. Carcinogenesis effects of gastric and duodenal refluxate on esophageal mucosa.
Zhonghua Neike Zazhi,2000,39:821-824
|
被引
1
次
|
|
|
|
9.
Liu SH. Ultrastructural investigation in esophageal mucosa of nonerosive gastroesophageal reflux disease.
Zhonghua Xiaohua Zazhi,2006,26:18-21
|
被引
1
次
|
|
|
|
10.
Oberg S. Determinants of intestinal metaplasia within the columnar-lined esophagus.
Archives of Surgery,2000,135:651-655,655-656
|
被引
2
次
|
|
|
|
11.
Martinez de Haro L. Intestinal metaplasia in patients with columnar lined esophagus is associated with high levels of duodenogastroesophageal reflux.
Annals of Surgery,2001,233:34-38
|
被引
4
次
|
|
|
|
12.
Tobey NA. Dilated intercellular spaces and shunt permeability in nonerosive acid-damaged esophageal epithelium.
American Journal of Gastroenterology,2004,99:13-22
|
被引
9
次
|
|
|
|
13.
Furuse M. Claudin-1 and -2:novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin.
Journal of Cell Biology,1998,141:1539-1550
|
被引
60
次
|
|
|
|
14.
Colegio OR. Claudin extracellular domains determine paracellular charge selectivity and resistance but not tight junction fibril architecture.
American Journal of Physiology-Cell Physiology,2003,284:C1346-C1354
|
被引
6
次
|
|
|
|
15.
Nusrat A. The coiled-coil domain of occludin can act to organize structural and functional elements of the epithelial tight junction.
Journal of Biological Chemistry,2000,275:29816-29822
|
被引
8
次
|
|
|
|
16.
Chen YH. Nonreceptor tyrosine kinase c-Yes interacts with occludin during tight junction formation in canine kidney epithelial cells.
Molecular Biology of the Cell,2002,13:1227-1237
|
被引
12
次
|
|
|
|
17.
Harhaj NS. Regulation of tight junctions and loss of barrier function in pathophysiology.
International Journal of Biochemistry & Cell Biology,2004,36:1206-1237
|
被引
31
次
|
|
|
|
18.
Furuse M. Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin at tight junctions.
Journal of Cell Biology,1994,127:1617-1626
|
被引
19
次
|
|
|
|
19.
Haskins J. ZO-3,a novel member of the MAGUK protein family found at the tight junction,interacts with ZO-1 and occludin.
Journal of Cell Biology,1998,141:199-208
|
被引
8
次
|
|
|
|
20.
Fanning AS. The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton.
Biological Chemistry,1998,273:29745-29753
|
被引
1
次
|
|
|
|
|