Intestinal microflora molecular markers of spleen-deficient rats and evaluation of traditional Chinese drugs
查看参考文献22篇
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文摘
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AIM: To find a rapid and efficient analysis method of gastrointestinal microflora in Pi-deficient (spleendeficient) rats and to evaluate traditional Chinese drugs. METHODS: Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) based assay was performed to examine changes of intestinal microflora in two Pi-deficienct animal models and to evaluate the efficacy of four traditional Chinese drugs as well as a probiotic recipe and another therapy in Pi-deficient rats. RESULTS: A molecular marker was identified for Pideficiency in rats. The pharmacodynamic evaluation system, including identified molecular markers (net integral area and abundance of DNA bands), Shannon's index for diversity of intestinal microflora, and Sorenson's pairwise similarity coefficient, was established. The four major clinical recipes of traditional Chinese drugs for Pi-deficiency in rats, especially at their medium dose (equivalence to the clinical dose), produced more pronounced recovery activities in Pi-deficient rats, while higher doses of these recipes did not show a better therapeutic effect but some toxic effects such as perturbation deterioration of intestinal microflora. CONCLUSION: Both fingerprint analysis and identified marker can show Pi-deficiency in rats and its difference after treatment. The identified molecular marker may be applied in screening for the active compounds both in relative traditional Chinese drugs and in pharmacodynamic study of Pi-deficiency in rats. |
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来源
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World Journal of Gastroenterology
,2009,15(18):2220-2227 【核心库】
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关键词
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Pi-deficiency
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Enterobacterial repetitive intergenic consensus-PCR
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Traditional Chinese medicine
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地址
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1.
School of Pharmacy,Shanghai Jiao Tong University, Shanghai, 200030
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Northern Institute for Cancer Research,Newcastle University, United Kingdom, Newcastle
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School of Pharmacy,Shenyang Pharmaceutical University, Liaoning, Shenyang, 110016
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语种
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英文 |
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文献类型
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研究性论文 |
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ISSN
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1007-9327 |
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学科
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内科学 |
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文献收藏号
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CSCD:3614421
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参考文献 共
22
共2页
|
|
1.
Kong J. A molecular biological method for screening and evaluating the traditional Chinese medicine used in Pi-deficiency therapy involving intestinal micro- flora.
Yazhou Chuantong Yiyao,2006,1:1-6
|
被引
1
次
|
|
|
|
|
2.
Zhu S. Experimental research on the effects of Jianpizhixie granules on the intestinal flora and small intestine mucosa in mice with diarrhea of splenic deficiency type.
Beijing Zhongyiyao Daxue Xuebao,2003,26:28-30
|
被引
1
次
|
|
|
|
|
3.
Hu J. Research regulation of traditional Chinese drugs SHENQU to alteration of intestinal flora mice and the intestines protective function.
Zhongguo Wei Shengtaixue Zazhi,2004,16:208-211
|
被引
1
次
|
|
|
|
|
4.
Ding WJ. Influence of Shenlinbaizhu Powder in enteric bacteria flora in mouse model with spleen-insufficiency syndrome.
Beijing Zhongyiyao Daxue Xuebao,2006,29:530-533
|
被引
3
次
|
|
|
|
|
5.
Daly K. Bacterial diversity within the equine large intestine as revealed by molecular analysis of cloned 16S rRNA genes.
FEMS Microbiology Ecology,2001,38:141-151
|
被引
16
次
|
|
|
|
|
6.
Vaahtovuo J. Bacterial composition of murine fecal microflora is indigenous and genetically guided.
FEMS Microbiology Ecology,2003,44:131-136
|
被引
3
次
|
|
|
|
|
7.
Versalovic J. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes.
Nucleic Acids Research,1991,19:6823-6831
|
被引
171
次
|
|
|
|
|
8.
Gillings M. Repetitive element PCR fingerprinting (rep-PCR) using enterobacterial repetitive intergenic consensus (ERIC) primers is not necessarily directed at ERIC elements.
Letters in Applied Microbiology,1997,25:17-21
|
被引
24
次
|
|
|
|
|
9.
Di Giovanni GD. Fingerprinting of mixed bacterial strains and BIOLOG gramnegative (GN) substrate communities by enterobacterial repetitive intergenic consensus sequence-PCR (ERIC-PCR).
Current Microbiology,1999,38:217-223
|
被引
24
次
|
|
|
|
|
10.
Wei G. ERIC-PCR fingerprinting- based community DNA hybridization to pinpoint genome-specific fragments as molecular markers to identify and track populations common to healthy human guts.
Journal of Microbiological Methods,2004,59:91-108
|
被引
24
次
|
|
|
|
|
11.
Van Driessche E. Prevalence,enumeration and strain variation of Arcobacter species in the faeces of healthy cattle in Belgium.
Veterinary Microbiology,2005,105:149-154
|
被引
2
次
|
|
|
|
|
12.
Zheng XW. Experimental study on effect of Buzhong Yiqi decoction on serum gastrin in spleen-qi de- ficiency rats.
Zhonghua Zhongyiyao Zazhi,2006,21:393-395
|
被引
1
次
|
|
|
|
|
13.
Qiu JF. Establishment of animal model of spleen deficiency in rats and therapeutic effects of traditional China medicine.
Shiyan Dongwu Kexue Yu Guanli,2006,23:13-15
|
被引
1
次
|
|
|
|
|
14.
Wang XM. Objective evaluation on spleen deficiency syndrome animal models.
Zhonghua Zhongyiyao Zazhi,2006,21:406-408
|
被引
1
次
|
|
|
|
|
15.
Peng Y. Orthogonal array design in optimizing ERIC-PCR system for fingerprinting rat's intestinal microflora.
Journal of Applied Microbiology,2007,103:2095-2101
|
被引
6
次
|
|
|
|
|
16.
Scanlan PD. Culture-independent analyses of temporal variation of the dominant fecal microbiota and targeted bacterial subgroups in Crohn's disease.
Journal of Clinical Microbiology,2006,44:3980-3988
|
被引
9
次
|
|
|
|
|
17.
McCracken VJ. Molecular ecological analysis of dietary and antibiotic-induced alterations of the mouse intestinal microbiota.
Journal of Nutrition,2001,131:1862-1870
|
被引
21
次
|
|
|
|
|
18.
Li F. Optimization of terminal restriction fragment polymorphism (TRFLP) analysis of human gut microbiota.
Journal of Microbiological Methods,2007,68:303-311
|
被引
7
次
|
|
|
|
|
19.
Liu YZ. Si- Jun-Zi decoction repairs mitochondrial damage of cells of liver myocardium,gastric mucosa and skeletal muscle in rats with spleen asthenia.
Zhongguo Linchuang Kangfu,2006,10:170-173
|
被引
5
次
|
|
|
|
|
20.
Ju BL. Study regulation of Chinese drugs Si Junzi Tang to alteration of intestinal flora mouse.
Mudanjiang Yixueyuan Xuebao,2003,24:4-6
|
被引
1
次
|
|
|
|
|
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