多孔导电膜刻蚀方法的改进及其在毛细管电泳蛋白进样浓缩中的应用
Improvement of Porous Electro-conductive Membrane Preparation and Its Application in Sample Injection Pre-concentration in Capillary Electrophoresis of Proteins
查看参考文献24篇
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文摘
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通过控制电压偏置方向,利用电渗流效应使毛细管多孔导电膜的刻蚀成功率得到显著提高.在刻蚀过程中于管中灌注偏碱性溶液,并在毛细管进口端与刻蚀池间施加正电压偏置.当刻蚀达到电通透时,在电渗流的作用下刻蚀自动停止,从而避免了过刻蚀.在含有2%～4%的葡聚糖的0.05 mol/L Tris-H_3PO_4 (pH 2.5)缓冲体系中,利用该多孔膜对溶菌酶和牛血清白蛋白实现了接近理论值的浓缩倍率.对酸碱性不同的蛋白浓缩效率的差别进行了讨论.利用电进样浓缩30 min使常规光度检测器(214 nm)对两个蛋白样品的检出限减小为1×10~(-11) mol/L. |
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其他语种文摘
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The success rate of porous conductive membrane preparation on fused silica capillary was greatly improved by control of voltage bias and electroosmosis effect.Basic buffer media was filled into the column,and a direct curvent voltage was applied between the capillary inlet and the etching reservoir during the etching process.The etching was stopped due to electroosmosis effect when electro-conductivity of the etching membrane occurred,and over etching was automatically avoided.The conductive membrane prepared was successfully used in on-line sample electro injection pre-concentration and capillary electrophoresis separation of model proteins of lysozyme and bovine serum albumin in 0.05 mol/L pH 2.50 Tris-H_3PO_4 with 2%-4% dextran,and a concentration factor close to the theoretical value was obtained.The difference of concentration factors for basic and acidic proteins was discussed.Detection limits for the model proteins were decreased to 1×10~(-11) mol/L with a routinely used photometric detector(214 nm wavelegnth) by electro-injection pre-concentration for 30 min. |
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来源
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分析化学
,2008,36(7):879-884 【核心库】
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关键词
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毛细管电泳
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多孔导电膜
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进样浓缩
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溶菌酶
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牛血清白蛋白
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光度检测
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地址
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东北大学分析科学研究中心, 辽宁, 沈阳, 110004
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0253-3820 |
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学科
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化学 |
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基金
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国家教育部留学回国人员科研启动基金
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文献收藏号
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CSCD:3333224
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参考文献 共
24
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