拟南芥Atfin1锌指蛋白的细胞定位
Cell Localization of Atfin1 Zinc Finger Protein in Arabidopsis
查看参考文献7篇
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文摘
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[目的]确定拟南芥锌指蛋白基因Atfin1的效应部位。[方法]用Trizol提取拟南芥叶片的总RNA,根据GeneBank数据库中Atfin1cDNA全序列设计引物,进行Atfin1的PCR扩增,克隆Atfin1基因,并进行同源性比较,构建pBI-Atfin1-EGFP融合表达载体,对Atfin1进行定位。[结果]克隆得到的拟南芥Atfin1蛋白氨基酸序列同紫花苜蓿Atfin1蛋白氨基酸序列的同源性达78.60%,在接近C-末端区域形成锌指结构的8个氨基酸残基高度保守,与周围的氨基酸残基共同形成典型的C_4HC_3基序。融合有Atfin1的绿色荧光蛋白只在细胞核区域表达,而未做融合的绿色荧光蛋白在整个细胞内呈弥散状分布。Atfin1没有典型的核定位信号,但仍然能够准确地在细胞核内表达。[结论]Atfin1在细胞核内表达,符合作为转录因子的表达特征。 |
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其他语种文摘
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[Objective] The study aimed to confirm the effector sites of zinc finger protein gene Atfin1 in arabidopsis.[Method] The total RNA was extracted from arabidopsis leaves by Trizol extraction.The primer was designed on the basis of complete sequence of Atfin1 cDNA in GeneBank database and the PCR amplification,clone and homologous comparison of Atfin1 were conducted.The fusion expression vector pBI-Atfin1-EGFP was constructed and used to localize Atfin1. [Result] The homology between amino acid sequence of cloned Atfin1 protein in arabidopsis and that in alfalfa reached 78.60% and the 8 amino acid residues forming zenic finger structure near C-terminal domain were highly conservative and formed typical C_4HC_3 motif together with peripheral amino acid residues.The green fluorescent protein infused with Atfin1 was only expressed in nuclear compartment and the green fluorescent protein witout Atfin1 distributed scatteredly in the whole cell.Atfin1 had no typical nuclear localization signal,but also could be expressed accurately in nuclear.[Conclusion] Atfin1 was expressed in nuclear,according with the expression characteristics of transcription factors. |
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来源
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安徽农业科学
,2008,36(19):8055-8056,8094 【扩展库】
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关键词
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锌指蛋白
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拟南芥
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细胞定位
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地址
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中国科学院西北高原生物研究所, 青海, 西宁, 810001
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0517-6611 |
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学科
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农学(农艺学) |
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基金
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中国科学院西部之光人才培养计划
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文献收藏号
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CSCD:3324961
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参考文献 共
7
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