药用植物的丛枝菌根接种方法研究
Inoculation Methods of AM Fungi in Medicinal Plant
查看参考文献48篇
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文摘
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本文借鉴菌根技术在农林业研究方面的研究成果，对丛枝菌根真菌在药用植物种植研究中的繁殖技术进行了分类总结。主要从药用植物AM真菌的培养方法及培养基质、不同孢子表面灭菌方法对侵染的影响、不同菌剂和寄主植物对侵染效果的影响、pH值对AM真菌侵染的影响、促生根细菌对AM真菌侵染效果的影响、信号物质对药用植物AM真菌培养的影响几个方面进行论述。结果发现丛枝菌根的影响因素复杂，寄主植物的种类、菌剂的种类、培养方法和条件等因素都对菌根孢子萌发和侵染有显著影响。结合药用植物的特殊性和研究实例，提出了中药植物接种丛枝真菌应综合考虑：药用植物接种菌根的目的、药用植物的生长特性、接种剂的特性、药用植物与菌根真菌的相互影响等因素，并在此基础上选择适当的方法和条件进行药用植物的接种实验。 |
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其他语种文摘
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With the development of CMM （Chinese Medicinal Materials） cultivation, cuhivar materials were used more than wild species. In order to overcome the continuous cropping obstacle, heavy metals exceed the permissible limits, medical plants disease and insect pest during the cultivation, AM （arbuscular mycorrhizal） fungi, which were associated widely with majority of plants and widely used in agriculture and forest as a biological technology, were developed in CMM cultivation. AM fungi were effect significant because they can form a symbiotic association in roots with a host plant. The host plant provided the fungus with soluble carbon sources, and the fungus provides the host plant with an increased capacity to absorb water and nutrients from the soil. AMF-colonized plants were generally more resistant to stresses caused by drought, salt, heavy metals or attack by pathogens. These positive effects of the fungi on the growth of plants often resulted from an improved nutrient supply. Inoculation methods of AM fungi in medicinal plants were different with the common plants, because secondary metabolites were considered to be more important in medicinal plant. Considering the particularity of medicinal plant, people should pay more attention in the process of inoculation, which included： inoculation methods and cultivation conditions, different surface sterilizing methods, different pH conditions, different host plants, and different AM fungus. PGPR （plant growth-promoting rhizobacteria）, plant signal compounds and temperature all have influence on the formation and function of Arbuscular mycorrhizas. CMM cultivation indicated AM had great influences to some medicinal plants, which included the secondary metabolite, the anti-adverse circumstance press, heavy metal pollution and the cultivation place soil restores and so on. Based on the particularity of CMM plant and research actuality, the following questions must be considered before selecting the proper methods and condition to inoculation： the purpose of experiment, the interaction between CMM plant and AM fungi, the characteristic of CMM plants and the characteristic of AM fungi. |
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来源
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资源科学
,2008,30(5):778-785 【核心库】
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关键词
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AM真菌
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中药栽培
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接种方法
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盆栽培养
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培养基培养
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地址
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中国中医科学院中药研究所, 北京, 100700
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1007-7588 |
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学科
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植物学;畜牧、动物医学、狩猎、蚕、蜂 |
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基金
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国家973计划
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国家自然科学基金
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国家科技支撑计划项目
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文献收藏号
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CSCD:3296010
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参考文献 共
48
共3页
|
|
1.
崔光红. 药用植物濒危与保护等级划分中的问题及其标准探讨.
中国中药杂志,2005,30(18):1474-1477
|
被引
2
次
|
|
|
|
|
2.
胡世林.
中国道地药材,1989
|
被引
46
次
|
|
|
|
|
3.
黄璐琦. 中药材道地性研究的现代生物学基础及模式假说.
中国中药杂志,2004,29(6):494-496
|
被引
60
次
|
|
|
|
|
4.
Philippine Vergeer. The effect of turf cutting on plant and arbuscular mycorrbizal spore recolonisation:Implications for heathland restoration.
Biological Conservation,2006(129):226-235
|
被引
1
次
|
|
|
|
|
5.
S E.
Read.Mycorrhizal Symbiosis(Second Edition),1997
|
被引
1
次
|
|
|
|
|
6.
Keunho Cho. Mycorrbizai symbiosis and response of sorghum plants to combined drought and salinity stresses.
Journal of Plant Physiology,2006(163):517-528
|
被引
1
次
|
|
|
|
|
7.
Meyer F H.
Distibution of Ectomycorrhizae in Native and Man-Made Forests,1973
|
被引
1
次
|
|
|
|
|
8.
刘润进.
《菌根学》,2007
|
被引
1
次
|
|
|
|
|
9.
Morandi D. Occurrence of phytoalexins and phenolic compounds in endomycorrhizal interactions and their potential role in hiological control.
Plant and Soil,1996,185:241-251
|
被引
13
次
|
|
|
|
|
10.
魏改堂. VA菌根真菌对荆芥的生长、营养吸收及挥发油合成的影响.
中国中药杂志,1991,16(3):139-142
|
被引
7
次
|
|
|
|
|
11.
魏改堂. VA菌根真菌对药用植物曼陀罗（Datura stramonium L.）生长,营养吸收及有效成分.
中国农业科学,1989,22(5):56-61
|
被引
25
次
|
|
|
|
|
12.
郭兰萍. 泡囊丛枝菌根（AM）对苍术生长发育及挥发油成分的影响.
中国中药杂志,2006,31(18):1491-1496
|
被引
20
次
|
|
|
|
|
13.
曾燕. 丛枝菌根及其在中药栽培中的应用.
世界科学技术一中医药现代化,2007,9(26):83-86
|
被引
6
次
|
|
|
|
|
14.
毕银丽. 丛枝菌根的双重培养方法及其菌丝际的建立.
菌物系统,2000(4):517-522
|
被引
9
次
|
|
|
|
|
15.
许学明. VA菌根真菌纯系增殖培养方法研究初报.
山东农业大学学报(自然科学版),1997(4):447-452
|
被引
4
次
|
|
|
|
|
16.
Jilicoeur M. Production of Glomus intraradices propagules an arboscular mycorrhizal fungus in an airlife bioreactor.
Biltenchnol Bioeng,1999(63):224-232
|
被引
1
次
|
|
|
|
|
17.
杨晓红. 丛枝菌根真菌培养方法研究进展.
菌物学报,2004,23(3):444-456
|
被引
9
次
|
|
|
|
|
18.
杨晓红. 丛枝菌根真菌的垂直平板定时转动培养及菌丝生长观察.
菌物系统,2001,20(31):358-361
|
被引
8
次
|
|
|
|
|
19.
Gaur A. Effects of the particle size of soilless substrates upon AM fungus inoculum production.
Mycorrhiza,2000,10(1):43-48
|
被引
16
次
|
|
|
|
|
20.
王幼珊. 盆栽基质及营养液对AM真菌接种剂繁殖的影响.
华北农学报,2001,16(4):81-86
|
被引
14
次
|
|
|
|
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