文摘
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[目的]采用反相高效液相色谱法对抱茎獐牙菜中当药黄素的含量进行测定。[方法]色谱柱为Kromasil C18(250min×4.60mm,5μm),流动相为甲醇-浓度0.02%磷酸水溶液,梯度洗脱,流速为1ml/min,检测渡长为260nm。[结果]当药黄素在0.92-4.60μg范围内线性关系良好,r=0.99950平均加样回收率为98.73%,RSD为0.31%。[结论]抱茎獐牙菜中当药黄素在花的部位含量最高。该测定方法适应性广,可用于测定抱茎獐牙菜中的当药黄素。 |
其他语种文摘
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[Objective] The research aimed to determine the content of swertisin in different parts of Swertia franchetiana H.Smith by reversed- phase high performance liquid chromatography. [Method] The analysis was performed on a Kromasil C18 (250 mm×4.60 mm, 5 μm). The mobile phase consisted of CH3OH and 0.02% phosphoric acid in water. The flow rate was 1 ml/min. The detection wavelength was at 260 nm. [Result] The linear range of swertisin was within 0.92- 4.60 μg(r=0.999 5). The average recovery was 98.73% ,RSD was 0.31%. [Conclusion] The content of swertisin was most high in flowers. |
来源
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安徽农业科学
,2008,36(8):3248-3249 【扩展库】
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关键词
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反相高效液相色谱法
;
抱茎獐牙菜
;
当药黄素
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地址
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1.
青海民族学院化学系, 青海, 西宁, 810007
2.
中国科学院西北高原生物研究所, 青海, 西宁, 810001
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语种
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中文 |
文献类型
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研究性论文 |
ISSN
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0517-6611 |
学科
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农作物 |
基金
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国家中西部重点项目(2001BA901A47)资助
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文献收藏号
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CSCD:3273699
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