不同纬度螅状独缩虫耐热能力及Hsp70 mRNA表达水平的比较
Comparative analysis of the Hsp70 mRNA expression and heat tolerance in two regional Carchesium polypinum at different latitudes
查看参考文献27篇
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文摘
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本文以游泳体的形成作为虫体对热激响应的指征,比较了不同纬度 (西安:东经10 8°98′,北纬34°25′;武汉:东经114°34′,北纬30°57′) 两地螅状独缩虫 (Carc hesium polypinum) 的耐热能力,发现武汉地区螅状独缩虫 (WH株) 形成游泳体的热激温度 ( 36℃) 高于西安地区 (XA株) 的33℃,说明前者的耐热能力较强;进一步利用实时PCR技术分析不同热激温度下两地螅状独缩虫热休克蛋白Hsp70 mRNA的表达变化发现,XA株Hs p70 mRNA热激后高表达水平的阈值温度为28℃,而WH株的为33℃,表明在螅状独缩虫中, Hsp70 mRNA表达的阈值温度与它们的耐热能力之间存在正相关.从而在分子水平阐明了螅状独缩虫WH株较XA株具有更高耐热能力的原因,这为研究纤毛虫野生种群热休克蛋白Hsp7 0 mRNA表达与栖息环境温度间的关系,进而了解原生动物温度适应的分子机理提供了线索. |
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其他语种文摘
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This study examined differences in heat tolerance in two populations of the ciliate Carchesium polypinum. Carchesium polypinum populations in Wuhan （WH strain） had higher thermal tolerance than those in Xi＇an （XA strain）, based on measurements of the threshold temperature under which the zooid （body having normal shape） transferred to the telotroch （body gone from the stalk and presented global shape under abominable conditions） was higher in the WH strain （36℃） than in XA strain （33℃）. Real-time PCR results showed that the WH strain had a higher threshold temperature for inducing high Hsp70 mRNA （33℃） than did the XA strain （28℃）. The correlation between the threshold temperature for Hsp70 mRNA and the thermal tolerance was oositive in C. polypinum. |
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来源
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动物学报
,2008,54(3):525-530 【核心库】
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关键词
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螅状独缩虫
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Hsp70 mRNA
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热激
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耐热能力
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实时PCR
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地址
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中国科学院水生生物研究所, 武汉, 430072
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0001-7302 |
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学科
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动物学 |
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基金
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国家自然科学基金
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文献收藏号
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CSCD:3265741
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