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黄花杓兰云南中旬居群遗传结构及克隆多样性的分析
Genetic Structure and Clonal Diversity of Cypripedium flavum (Orchidaceae) Populations from South-West China

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蔡凝枫 1   严宁 1   胡虹 1   刘涛 2  
文摘 采用AFLP分子标记方法对云南中甸的6个黄花杓兰(Gypripedium flavum)居群进行了遗传多样性水平、遗传结构及克隆多样性研究,其中两个居群用样方取样法分析其克隆空间结构。POPGENE软件分析结果表明:两组引物共产生104个位点,其中86个为多态位点,多态位点百分率为82.69%。黄花杓兰具有丰富的遗传变异(物种水平上,He=0.2884,Ho=0.4312;居群水平上,P=64.59%,He=0.2449,Ho=0.3606),黄花杓兰居群的遗传分化不大(Gst=0.154),居群间基因交流较为频繁(Nm=2.7460)。居群的克隆多样性水平高(D=0.9638—0.9960,G/N=0.83—0.96),同一克隆的分株相邻,克隆生长延伸范围狭窄。黄花杓兰居群之所以保持较高水平的遗传多样性,可能与其既能通过实生幼苗增加基因的杂合度,又能通过无性分株把杂合体固定下来有关。
其他语种文摘 Six populations of Cypripedium flavum were studied on their genetic diversity, genetic structure, and clonal diversity in Zhongdian County, Yunnan Province (South-west China). Using POPGENE software, 86 polymorphic loci was obtained using two AFLP primer combinations, and the proportion of polymorphic loci was 82.69%. A relatively high level of genetic diversity was revealed: He =0.2884, Ho =0.4312 (at species level); P=64.59%, He =0.2449, Ho =0.3606 (at population level). Genetic differentiation among populations was not high ( Gst = 0.154). A relatively high level of gene flow was obtained among populations. Clonal diversity of populations was high ( D = 0.9638-0.9960, GIN = 0.83-0.96), and ramets of same genotype were adjacent. C .flavum was found with high level of genetic diversity. This may be explained by gene heterozygesity increasing by seedling recruitment and maintaining by clonal reproduction.
来源 云南植物研究 ,2008,30(1):69-75 【核心库】
关键词 黄花杓兰 ; 遗传多样性 ; 遗传结构 ; 克隆 ; AFLP
地址

1. 中国科学院昆明植物研究所, 云南, 昆明, 650204  

2. 云南农业大学农学与生物技术学院, 云南, 昆明, 650201

语种 中文
文献类型 研究性论文
ISSN 0253-2700
学科 分子生物学
基金 国家自然科学基金 ;  中国科学院知识创新工程重要方向项目
文献收藏号 CSCD:3205518

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引证文献 6

1 尤卫艳 光皮桦AFLP分子标记体系的建立 生物技术,2008,18(6):42-45
被引 6

2 孙叶迎 长白山区不同花色杓兰属植物遗传多样性的ISSR分析 西北农林科技大学学报. 自然科学版,2014,42(9):137-143
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