云南南部不同种源地小桐子遗传多样性的ISSR分析
Genetic Diversity of Jatropha curcas （Euphorbiaceae） Collected from Southern Yunnan, Detected by Inter-simple Sequence Repeat （ISSR）
查看参考文献16篇
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文摘
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应用ISSR分子标记方法对采自云南的8个居群的小桐子（Jatropha curcas）共158个个体进行遗传多样性分析。8个ISSR引物共扩增到了67个位点，其中61个是多态性位点。分析结果表明：（1）云南小桐子的遗传多样性水平很高。在物种水平上，平均每个位点的多态位点百分率PPB=91．04％，有效等位基因数Ne=1．5244，Nei’s基因多样性指数垃=0．3070，Shannon多样性信息指数Ho=0．4618；在居群水平上，PPB=55．04％，N_e=1．3826，H_e=0．2171，Shannon多样性信息指数Ho=0．3178。（2）居群间的遗传分化低于居群内的遗传分化。基于Nei’s遗传多样性分析得出的居群间遗传多样性分化系数Gst=0．2944。AMOVA分析显示：云南小桐子的遗传变异主要存在于居群内，占总变异的63．50％，居群间的遗传变异占36．50％。（3）居群间的地理距离及遗传一致度并不存在相关性。鉴于以上指标，我们推测云南小桐子可能来自不同的地区。 |
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其他语种文摘
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The genetic diversity of 158 individuals from eight semi-wild populations from Yunnan Province was estimated using ISSR method （8 primers）. The results revealed an extraordinarily high level of genetic diversity （at species level, percentage of polymorphic loci PPB = 91.04%, effective number of alleles N_e = 1.5244, Nei＇s （1973） gene diversity He =0.3070, and Shannon＇s information index Ho =0.4618; at population level, PPB = 55.04%, Ne = 1.3826, Nei＇s （1973） gene diversity H_e = 0.2171, and Shannon＇s information index Ho = 0.3178）. The level of genetic differentiation between populations is lower than that among populations. The low level of genetic differentiation among populations was detected, based on Nei＇s genetic diversity analysis （29.44%）, and AMOVA （36.50%）. ]here is no associations between geographical distance and genetic identity. We suggest that Jatropha curcas of Yunnan Province might not be introduced from the same place. |
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来源
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云南植物研究
,2007,29(6):619-624 【核心库】
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关键词
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小桐子
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遗传变异
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ISSR
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地址
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1.
中国科学院昆明植物研究所, 云南, 昆明, 650204
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中国科学院西双版纳热带植物园, 云南, 勐腊, 666303
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0253-2700 |
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学科
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分子生物学 |
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基金
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国家科技基础条件平台建设项目
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云南省自然科学基金
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文献收藏号
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CSCD:3042299
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