Pb~(2+)胁迫对绿球藻(Chlorococcum sp.)的影响研究
The Effects of Pb~(2+) Stress on Chlorococcum sp
查看参考文献29篇
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文摘
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研究了不同Pb~(2+)浓度(0.1、1、10、50、100、200、400 mg/L)处理对绿球藻(Chlorococcum sp.)生长、形态结构及生理特性的影响.与对照BG11培养的绿球藻比较,Pb~(2+) 浓度≤50 mg/L条件下培养的绿球藻细胞壁无明显增厚,色素变化不大;而暴露到Pb~(2+) 浓度＞50 mg/L条件下培养,绿球藻细胞壁明显增厚,蛋白核消失.低浓度Pb~(2+)(0.1～10 mg/L)对绿球藻生长基本没有影响;浓度在50 mg/L时,绿球藻仍能维持一定的生长速率;但当Pb~(2+) 浓度≥100 mg/L时,绿球藻的生长受到显著抑制.绿球藻的Chl a+Chl b以及Chl a含量均随培养基中Pb~(2+) 浓度的升高而逐渐减少.绿球藻净光合作用强度随培养基中Pb~(2+) 浓度的增大而逐渐降低,Pb~(2+) 浓度≥100 mg/L时净光合作用强度检测不到;当Pb~(2+)浓度＜50 mg/L时,绿球藻呼吸作用强度逐渐升高,之后呼吸作用强度逐渐降低.在实验的浓度下,绿球藻的丙二醛(MDA)含量、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性都随培养基中Pb~(2+) 浓度的升高而逐渐增强;过氧化氢酶(CAT)则随Pb~(2+)浓度的增大酶活性先升高后降低.当Pb~(2+) 浓度≤100 mg/L 时,绿球藻对Pb~(2+) 的去除率都在95%以上;之后逐渐降低,浓度到400 mg/L时仍然达56.7%.结果显示,绿球藻是一种耐受Pb~(2+) 胁迫的藻类,对铅的去除率也高,可以应用于含铅污水的处理. |
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其他语种文摘
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The effects of different concentration Pb~(2+)(0.1,1,10,50,100,200,400 mg/L) in BG11 on the growth,morphostructure and physiological characteristics of Chlorococcum sp.were studied.Compared with the cell cultured in BG11,the cell cultured in low concentrations(≤50 mg/L) showed few changes in pigment and thickness of cell wall;the cell wall of the cell cultured in high concentrations of PbPb~(2+)(>50 mg/L)became thicker,and the pigment decreased and the pyrenoid disappeared.When the concentrations of Pb~(2+) were 0.1-10 mg/L,the growth of Chlorococcum sp.showed no obvious difference compared with the control;when the concentration of Pb~(2+) was 50 mg/L,Chlorococcum sp.could maintain certain growth rate yet;however,when the concentration of Pb~(2+) was higher than 100 mg/L,the growth of Chlorococcum sp.was inhibited markedly.The contents of Chl a+Chl b or Chl a decreased gradually with the increase of the concenrations of Pb~(2+) in the medium.The photosynthesis of Chlorococcum sp.decreased gradually with the increase of Pb~(2+) concentrations,when the concentration of Pb~(2+) was higher than 100 mg/L,the photosynthesis of Chlorococcum sp.could not be detected;when the concentration of Pb~(2+) was less than 50 mg/L,the respiration of Chlorococcum sp.increased gradually with the increase of Pb~(2+) concentrations,and when the concentration of Pb~(2+) was higher than 50 mg/L,they decreased gradually with the increase of Pb~(2+) concentrations.The content of malondiadehyde and activities of superoxide dismutase and peroxidase increased gradually with the increase of Pb2+ concentrations,and the activity of catalase increased at beginning and then decreased with the increase of Pb~(2+) concentrations.When the concentration of Pb~(2+) was ≤100 mg/L,the removal rate of Chlorococcum sp.on Pb~(2+) was >95%,and it was up 56.7% yet when the concentration of Pb~(2+) was 400 mg/L.The results demonstrated that the Chlorococcum sp.could be applied to the treatment of wastewater containing Pb~(2+),because the Chlorococcum sp.could endure the stress of Pb2+ and was of high removal rate on Pb~(2+). |
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来源
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武汉植物学研究
,2007,25(5):521-526 【核心库】
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关键词
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绿球藻
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Pb~(2+)
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生长
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生理特性
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细胞结构
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地址
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中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉, 430072
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语种
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中文 |
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文献类型
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简报 |
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ISSN
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1000-470X |
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学科
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环境科学基础理论 |
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基金
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国家863计划
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湖北省教育厅重点项目
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湖北省黄石市科技攻关项目
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文献收藏号
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CSCD:2944327
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