低温保存技术在顽拗性种子种质保存中的利用
Cryopreservation of recalcitrant seed germplasm
查看参考文献54篇
|
文摘
|
由于顽拗性种子不耐脱水且对低温敏感,常规保存方法难以达到长期保存的目的.因此,(超)低温保存顽拗性种子种质是最理想的方法.顽拗性种子的低温保存,应用较多的是玻璃化法和两步法.诸多因素影响着低温保存的成败,如种子或胚的含水量水平、溶液低温保护剂效应、降温冰冻与解冻方式、水合过程以及后培养等,这些需深入探索与解决.除顽拗性种子脱水耐性和低温敏感性机理外,植物细胞的冻害和抗冻机理也亟需探明,以便找到最佳冷冻方法,制定长期保存种质基因的最佳方案. |
|
其他语种文摘
|
Being sensitive to desiccation and low temperature,the recalcitrant seeds can not be stored with conventional methods. However,cryopreservation has been considered as the most effective means. As far as cryostorage of recalcitrant seeds is concerned,vitrification and two-step freezing have been applied in many studies. Unfortunately,there are some factors,including water content of seeds or embryonic axes, effects of cryopretectants in solutions,cooling rate and thawing,rehydration and after-thawing culture that influence succeeding in cryopreservation. To produce normal plants after cryopreservation of recalcitrant seeds or embryonic axes,a carefully defined sequence of manipulations is needed to be solved. Besides the mechanisms of desiccation tolerance and sensitivity to low temperatures,the freezing and antifreezing mechanisms of plant cells are also ascertained imminently in order to dig out the most effective long-term storage method through seed-gene bank.
|
|
来源
|
广西植物
,2007,27(5):759-764 【核心库】
|
|
关键词
|
顽拗性种子
;
(超)低温保存
;
玻璃化法
;
两步法
;
种子含水量
;
脱水方式
|
|
地址
|
中国科学院,昆明植物研究所, 昆明, 650204
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1000-3142 |
|
学科
|
植物学 |
|
基金
|
国家自然科学基金
;
国家科技部平台建设项目
|
|
文献收藏号
|
CSCD:2932205
|
参考文献 共
54
共3页
|
|
1.
Al Ababneh S. Cryopreservation of sour orange(Citrus aurantium)shoot tips.
In vitro Cell Develop Bio-Plant,2002,38:602-607
|
被引
1
次
|
|
|
|
|
2.
Assy-Bah B. Cryopreservation of mature embryos of cacaonut and subsequent regeneration of plantlets.
Cryoletters,1992,13:117-126
|
被引
2
次
|
|
|
|
|
3.
Becwar MR. Dehydration effects on freezing characteristics and survival in liquid nitrogen of desiccation-tolerant and desiccation-sensitive seeds.
J Am Soc Hort Sci,1983,108:613-618
|
被引
4
次
|
|
|
|
|
4.
Berjak P. Protector of the seeds:seminal reflections from southern Africa.
Science,2005,307:47-49
|
被引
3
次
|
|
|
|
|
5.
Berjak P. Effects of developmental status and dehydration rate on characteristics of water and desiccation-sensitivity in recalcitrant seeds of Camellia sinensis.
Seed Sci Res,1993,3:155-166
|
被引
17
次
|
|
|
|
|
6.
Bonner FT. Responses to drying of recalcitrant seeds of Quercus nigra.
Ann Bot,1996,78:181-187
|
被引
10
次
|
|
|
|
|
7.
Chandel KPS. Desiccation and freezing sensitivity in recalcitrant seeds of tea cocoa and jackfruit.
Ann Bot,1995,76:443-450
|
被引
12
次
|
|
|
|
|
8.
Channuntapipat C. Cryopreservation of in vitro almond shoot tips by vitrification.
J Hort Sci Biotech,2000,75(2):228-232
|
被引
12
次
|
|
|
|
|
9.
Gonzalez-Benito ME. Effects of seed cryopreservation and priming on germination in several cultivars of Apinum graveolens.
Ann Bot,1995,75:1-4
|
被引
1
次
|
|
|
|
|
10.
Hor YL. Effects of dehydration on freezing characteristics and survival in liquid nitrogen of three recalcitrant seeds.
Pertanika,1990,13:309-314
|
被引
2
次
|
|
|
|
|
11.
Hirano T. Cryopreservation of immature seeds of Bletilla striata by vitrification.
Plant Cell Reports,2005,23:534-539
|
被引
10
次
|
|
|
|
|
12.
简令成. 低温生物学与植物种质的长期保存.
植物学通报,1988,5(2):65-68
|
被引
7
次
|
|
|
|
|
13.
Langis R. Cryopreservation of carnation shoot tips by vitrification.
Crybiology,1990,27:657-658
|
被引
6
次
|
|
|
|
|
14.
Litvan GG. Mechanism of cryounjury in biological systems.
Cryobiology,1972,9(3):182-191
|
被引
1
次
|
|
|
|
|
15.
马锋旺. 杏细胞悬浮培养物超低温保存有关因素的研究.
农业生物技术学报,1999,7(1):10-16
|
被引
3
次
|
|
|
|
|
16.
Matsumoto T. Cryopreservation of in vitro-grown apical meristems of wasabi(Wasabia japonica)by vitrification and subsequent high plant regeneration.
Plant Cell Reports,1994,13:442-446
|
被引
12
次
|
|
|
|
|
17.
Matsumoto T. Cryopreservation of in vitro-grown apical meristems of lily by vitrification.
The Plant Cell,1995,41:237-241
|
被引
1
次
|
|
|
|
|
18.
Matsumoto T. Cryopreservation of in vitro-grown apical meristems of hybrid statice by three different procedures.
Sci Hort,1998,76:105-114
|
被引
4
次
|
|
|
|
|
19.
Meryman HT. Cryoprotective agents.
Cryobiology,1971,8(2):173-183
|
被引
15
次
|
|
|
|
|
20.
Normah LN. Desiccation and cryopreservation of embryonic axes of Hevea brasiliensis.
Muell-Arg Pertanika,1986,9:299-303
|
被引
1
次
|
|
|
|
|
了解气象卫星更多信息，请访问