鱼类谷胱甘肽转移酶基因cDNA克隆及其序列分析
Cloning and the sequence analysis of the fish glutathione transferase Pi gene
查看参考文献15篇
文摘
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采用RT-PCR方法,从鲢、鲤、鲫3种鱼类肝脏总RNA中克隆出了谷胱甘肽转移酶Pi型(GSTPi)cDNA序列,推导了其编码的氨基酸序列。3种鱼类GSTPi的ORF全长627bp,编码208个氨基酸。翻译起始密码均为ATG,终止密码子均为TGA。鱼类与哺乳动物、两栖类爪蟾以及节肢动物丝虫之间GSTPi氨基酸序列相似度平均值分别为50%、33%、15%左右。5种鱼类之间的氨基酸序列相似度较大,其中鲤科鱼类之间平均为85%左右。我们以GSTPi为分子标记,用最大简约数法(MP)构建了13个物种的系统进化树,识别出两个大的单系类群:哺乳类组成类群一(bootstrap 100);鱼类组成类群二(bootstrap 93)。通过比较鱼类与哺乳类GSTPiN末端和c末端功能域的氨基酸组成差异,探讨了淡水鱼类GSTs承担较强的微囊藻毒素去毒能力的可能分子机制。
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其他语种文摘
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Using RT-PCR method, the glutathione transferase Pi cDNAs were cloned from Cyprinus carpio, Hypophthalmichthys molitrix, and Carassius auratus. The open reading frames (ORFs) from the 3 fishes were 627 bp long (encoding for 208 amino acids) with the initial code ATG and the terminal code TGA. The sequence similarity was 50% between fish and mammals, 33% between fish and amphibian, and 15% between fish and arthropoda, respectively. The sequence similarity was big among fishes, and the average value of the 4 cyprinids was about 85%. Phylogenetic tree was constructed for 13 species based on GST Pi amino acid sequences using MP (Maximum Parsimony) method. Two major clusters were recognized: cluster one consisted of Mammals (bootstrap 100) and cluster two consisted of fishes (bootstrap 93). Based on the sequences analyses of N/C domain of GST Pi, we proposed the detoxification mechanism of freshwater fishes that were thought to have stronger tolerance to microcystins.
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来源
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遗传
,2007,29(3):349-354 【核心库】
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关键词
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谷胱甘肽转移酶Pi型
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鲢
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鲤
;
鲫
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地址
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1.
华中农业大学水产学院, 农业动物遗传育种与繁殖教育部重点实验室, 武汉, 430070
2.
中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉, 430072
3.
华中农业大学水产学院, 农业动物遗传育种与繁殖教育部重点实验室, 430070
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语种
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中文 |
文献类型
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研究性论文 |
ISSN
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0253-9772 |
学科
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分子生物学 |
基金
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国家自然科学基金
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华中农业大学科研启动项目
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华中农业大学大学生科技创新基金(SRF)
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文献收藏号
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CSCD:2871455
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