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马铃薯Alfin1-like锌指蛋白基因克隆与序列分析
Cloning and Sequencing of an Alfin1-like Zinc Finger Protein Gene in Solanum tuberosum
查看参考文献9篇
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文摘
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利用紫花苜蓿盐胁迫相关锌指结构蛋白Alfin1基因cDNA序列,通过电子克隆在GenBank中对马铃薯同源EST序列进行查询比较和拼接,获得了1个含有完整编码区的cDNA序列,并通过RT-PCR成功获得了该序列.获得的全长cDNA序列中包含1个747 bp的最大读码框,编码248个氨基酸,将其命名为Stfin1.氨基酸序列分析表明存在典型的Cys4-His-Cys3锌指结构,与Alfin1一致性达93.09%.从结构分析结果推测Stfin1与Alfin1在功能上具有一定的相关性. |
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其他语种文摘
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A full length cDNA sequence was obtained by in silico cloning. After searching against potato EST database in GenBank using Alfinl cDNA sequence from alfalfa. The native cDNA sequence denoted as Stfinl (Solarium tuberosum Alfinl-like proteinl) was isolated with RT-PCR amplification. Stfinl contains a maximum open reading frame of 747 bp encoding a peptide with 248 amino acids and a stop codon. The Stfinl-deduced amino acid sequence showed 93. 09 % identity and shared a conserved typical Cys4-His-Cys3 zinc finger motif with Alfinl. Its sequence conservation and structure similarity with Alfinl implied the functional correlation between Stfinl and Alfinl in development processes and responses to abiotic stresses. |
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来源
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西北植物学报
,2007,27(4):657-661 【核心库】
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关键词
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锌指蛋白
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电子克隆
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马铃薯
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地址
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1.
中国科学院西北高原生物研究所, 西宁, 810001
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中国科学院研究生院, 北京, 100049
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-4025 |
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学科
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分子生物学;农作物 |
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基金
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中国科学院西部之光人才培养计划
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文献收藏号
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CSCD:2786787
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参考文献 共
9
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