黄河裸裂尻鱼群体遗传结构和Cyt b序列变异
PHYLOGENETIC STRUCTURE OF SCHIZOPYGOPSIS PYLZOVI POPULATIONS FROM MITOCHONDRIAL CYTOCHROME B GENE SEQUENCE VARIATIONS
查看参考文献21篇
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文摘
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测定了来自黄河上游和柴达木盆地托索湖的裸裂尻鱼共16个个体的Cyt b基因全序列（1141bp），探讨了种群结构和遗传多样性。用MEGA2．1软件分析了碱基组成和序列变异；以青海湖裸鲤、花斑裸鲤和极边扁咽齿鱼为外类群，用PAUP＊4．0b10程序构建了单倍型NJ树；用Arlequin Ver．2000程序计算了群体间遗传变异值（Fst）和Nm值以及群体分化概率值。结果显示，来自柴达木水系托索湖的裸裂尻鱼没有形成单系群，Fst=0．204（P〈0．05），Nm=1．95。初步判断，黄河和柴达木水系托索湖的裸裂尻鱼未显著分化，支持将柴达木裸裂尻鱼（Schizopygopsis kessleri）归并人黄河裸裂尻鱼（Schizopygopsis pylzovi）的形态学结果。两种群核苷酸多样度（π）分别为0．0012和0．0026，表现为较低水平。根据校正的分子钟推测，黄河和托索湖裸裂尻鱼群体分歧时间为距今7万年左右的更新世末期，结合地理分布的资料和古地质事件，对黄河裸裂尻鱼群体分布水系间的历史联系进行了分析。 |
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其他语种文摘
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The complete mtDNA cytochrome b gene sequence （1141bp） was sequenced for 16 Schizopygopsis from the upper reach of the Yellow River and the Tuosuo lake of the Qaidam Basin. The sequence compositions and variations were analyzed using MEGA2.1 software. The Gymnocypris przewalskii and Gymnocypris eckloni and Platypharodon extremus were selected as outgroup, and one molecular phylogenetic tree of 5 haplotypes was constructed using NJ in PAPU ＊ 4.0b10 software. The monophyly of the populations of the Schizopygopsis from either the Yellow River or the Tuosuo lake was not supported. The genetic variability （Fst） and the value of exact test of differentiation between the populations calculated with Arlequin Ver. 2000 software were 0.204（ P 〈 0.05）and 0.0532 （P 〉 0.05）, respectively. The Nm value deduced from Fst between the populations was 1.95. These data suggested that the populations of the Schizopygopsis from the Yellow River and the Tuosuo lake have not evolved to two different species. Our results support the morphological classification that merging the SchizoFygopsis kessleri into Schizopygopsis pylzovi. The nucleotide diversity （π） exhibited by two populations was low （0.0012 - 0.0026）. Inferred from the molecular evolution rate, the divergent time of the S. pylozovi from the Yellow River and the Tuosuo lake was about 70,000 years ago in the late Pleistocene. The faunal connection for population of the in historical was analyzed according to the phylogeography of population distributions and paleo-geographical events. |
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来源
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水生生物学报
,2006,30(2):129-133 【核心库】
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关键词
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黄河裸裂尻鱼
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柴达木裸裂尻鱼
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遗传结构
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遗传多样性
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Cyt b
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地址
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1.
中国科学院水生生物研究所, 武汉, 430072
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西北农林科技大学动物脂肪沉积与肌肉发育实验室, 杨凌, 712100
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-3207 |
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学科
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动物学 |
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基金
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国家自然科学基金项目
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中国科学院知识创新工程重要方向项目
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文献收藏号
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CSCD:2606356
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