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新疆天山雪莲体胚诱导与分化研究
Induction and Differentiation of Somatic Embryos of Saussurea involucrata
查看参考文献8篇
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文摘
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以新疆天山雪莲的叶片为外植体，分别用不同配方培养基诱导愈伤组织，后进行体胚诱导和分化培养形成再生雪莲植株。结果表明，诱导愈伤组织的最适培养基为MS＋2，4-D0．5mg／L＋BA1．5mg／L，诱导率可达到100％；愈伤组织转移至MS＋2，4-D0．5mg／L＋BA1．5mg／L培养基进行继代培养，增殖后的愈伤组织转移到MS＋2，4-D0．2mg／L的液体培养基后成功诱导出雪莲体胚，出胚率达40％；将体胚接至MS＋ABA0．5mg／L培养基后，结果分化生长出大量的再生雪莲幼苗。 |
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其他语种文摘
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The leaves of Saussurea involucrata as the explants were cultured and induced to form calli on the media prepared according to different formulae;The calli were cultured and induced to form somatic embryos, which were cultured to differentiate into regeneration plantlets of S. involucrata. It was shown that the optimal medium for callus induction was MS＋0.5 mg/L 2,4-D＋1.5 mg/L BA and its callus induction rate was as high as 100% ;The calli were transferred and secondarily cultured on the medium of MS＋0.5 mg/L 2,4-D＋1.5 mg/L BA and then the propagated calluses were transferred, cultured and induced to form somatic embryos of S. involucrata on the liquid medium of MS＋0.2 mg/L 2,4-D and the somatic embryo induction rate was as high as 40% ;The somatic embryos were inoculated on the medium of MS＋0.5 mg/L ABA and then differentiated into great numbers of regeneration plantlets of S. involucrata. |
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来源
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西北植物学报
,2006,26(7):1351-1354 【核心库】
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关键词
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新疆天山雪莲
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愈伤
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体胚
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地址
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中国科学院新疆理化技术研究所, 乌鲁木齐, 830011
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-4025 |
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学科
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生物工程学(生物技术);植物学 |
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基金
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中国科学院知识创新工程西部行动计划项目
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文献收藏号
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CSCD:2510967
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参考文献 共
8
共1页
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被引
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被引
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7.
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