镉和铜对嗜热四膜虫金属硫蛋白基因的诱导表达
Expression of metallothionein gene induced by cadmium and copper in Tetrahymena thermophila
查看参考文献36篇
文摘
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本文在荧光定量PCR优化的基础上,利用该技术考察了不同浓度的重金属镉和铜对嗜热四膜虫金属硫蛋白基因(MTT1)诱导表达的变化规律。结果表明:MTT1基因的表达对镉离子的诱导更灵敏,且在一定阈值浓度(≤35.2μmol/L)范围内,镉离子浓度升高会增加MTT1基因表达量,超过该阈值后表达量迅速下降;镉与铜同时诱导时MTT1基因的表达情况与镉单独诱导的类似,但阈值浓度减小为22μmol/L,表明二者的联合毒性为协同作用。镉离子浓度低于22μmol/L时,与铜离子的共同作用会大大增加MTT1基因的表达量,从而增强了四膜虫的解毒能力[动物学报51(6):1115-1121,2005]。 |
其他语种文摘
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Heavy metal pollution has become the major concern of many countries, and the key to control the matter calls for good model organisms as early-warning indicators, effective target genes as molecular markers, and a sensitive method studying differential expressions of the genes. To study the potential molecular mechanisms of cadmium and copper combined toxicity, the unicellular protozoa Tetrahymena thermophila was chosen for its fast growth and sensitivity to pollutants in water ecosystem, and a method of real-time fluorescent quantitative PCR (Q-PCR) was applied which is effective, accurate and has been widely used in molecular field. In this article, the Q-PCR method was optimized, on the basis of which tests were designed to study the relative expression of metallothionein gene ( MTT1 ) in T. thermophila treated with different concentrations of heavy metals cadmium and copper. The results suggested that: MTT1 gene was more sensitive to cadmium induction compared to copper, and in a certain threshold scale (435.2 μmol/L), raising the cadmium concentrations increased the MTT1 expression level, but made it decreased while beyond the threshold; the expression rule of MTT1 induced by both cadmium and copper was similar to that treated with cadmium only, but then the threshold down to 22 μmol/L, indicating that combined toxicity of cadmium and copper in T. thermophila were synergism. When treating concentration was lower than 22 μmol/L, cadmium cooperated with 244 μmol/L copper greatly increased the expression level of MTT1, hence enhancing the detoxification ability of Tetrahymena [ Acta Zoologica Sinica 51 (6): 1115-1121, 2005]. |
来源
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动物学报
,2005,51(6):1115-1121 【核心库】
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关键词
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嗜热四膜虫
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金属硫蛋白基因
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实时荧光定量PCR
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地址
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中国科学院水生生物研究所, 湖北, 武汉, 430072
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语种
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中文 |
文献类型
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研究性论文 |
ISSN
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0001-7302 |
学科
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动物学 |
基金
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中国科学院水生生物研究所淡水生态与生物技术国家重点实验室基金
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文献收藏号
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CSCD:2164089
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参考文献 共
36
共2页
|
1.
Abdel-Mageed AB. A review of the biochemical roles.
Zinc.Vet.Hum.Toxicol,1990,32:34-39
|
被引
1
次
|
|
|
|
2.
Ait-Oukhatar N. application to zinc metabolism.
Ann.Biol.Clin,1996,54:87-90
|
被引
2
次
|
|
|
|
3.
Akintola DF. Development of an enzyme-linked immunosorbent assay for human metallothionein-1.
J.Lab.Clin.Med,1995,126:119-127
|
被引
3
次
|
|
|
|
4.
Beattie JH. Strategies for the qualitative and quantitative analysis of metallotionein isoforms by capillary electrophoresis.
Talanta,1998,46:255-270
|
被引
4
次
|
|
|
|
5.
Beattie JH. Metallothionein overexpression and resistance to toxic stress.
Toxicology Letters,2005,157:69-78
|
被引
1
次
|
|
|
|
6.
Boldrin F. Metal interaction and regulation of Tetrahymena pigmentosa metallothionein genes.
Protist,2002,153:283-291
|
被引
5
次
|
|
|
|
7.
Chowdhury BA. Biological and health implications of toxic heavy metal and essential trace element interactions.
Prog.Food.Nutr.Sci,1987,11(1):55-113
|
被引
1
次
|
|
|
|
8.
Dabrio M. Recent developments in quantification methods for metallothionein.
Journal of Inorganic Biochemistry,2002,88:123-134
|
被引
19
次
|
|
|
|
9.
Dallinger R. Spectroscopic characterization of metallothionein from the terrestrial snail Helix pomatia.
Eur.J.Biochem,2001,268:4126-4133
|
被引
1
次
|
|
|
|
10.
Dondero F. Biochemical characterization and quantitative gene expression analysis of the multi-stress inducible metallothionein from Tetrahymena thermophila.
Protist,2004,155:157-168
|
被引
3
次
|
|
|
|
11.
Ferrarello C. a comparison with the conventional anion-exchange chromatography.
Analysis,2000,28:351-357
|
被引
1
次
|
|
|
|
12.
Fillingham JS. Analysis of expressed sequence tags (ESTs) in the ciliated protozoan Tetrahymena thermophila.
J.Eukaryot.Microbiol,2002,49:99-107
|
被引
4
次
|
|
|
|
13.
Gabert J. Standardization and quality control studies of 'real-time' quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia-A Europe Against Cancer Program.
Leukemia,2003,17:2318-2357
|
被引
62
次
|
|
|
|
14.
Gutierrez JC. Ciliates as a potential source of cellular and molecular biomarkers/biosensor.
Europ.J.Protistology,2003,39:461-467
|
被引
5
次
|
|
|
|
15.
Hamer DH. Metallothionein.
Annu.Rev.Biochem,1986,55:913-951
|
被引
39
次
|
|
|
|
16.
Heid CA. Real time quantitative PCR.
Genome Res,1996,6:986-994
|
被引
326
次
|
|
|
|
17.
K(a)gi JHR. Evolution.
Metallothionein Ⅲ,1993:29-55
|
被引
1
次
|
|
|
|
18.
Klein D. Quantification of oxidized metallothionein in biological material by a Cd saturation method.
Anal.Biochem,1994,221:405-409
|
被引
2
次
|
|
|
|
19.
Lee KF. Generation and characterization of hammerhead ribozymes targeting rodent metallothionein-Ⅰ and Ⅱ ribonucleic acid.
Toxicology Applied Pharmacology,1999,161:294-301
|
被引
1
次
|
|
|
|
20.
Livak KJ. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method.
Methods,2001,25:402-408
|
被引
8378
次
|
|
|
|
|