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新疆雪莲新的内切几丁质酶类冷诱导基因的分离、克隆和测序
Isolation, Molecular-cloning and Sequencing of a New Cold-responsive Endochitinase Gene in Saussurea involucrata Kar. et Kir.
查看参考文献7篇
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文摘
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通过RT-PCR技术，从经低温驯化的新疆雪莲的叶片中分离克隆了1个新的基因。实验证明，此基因的转录是由低温诱导激活，且随着雪莲低温驯化时间的推移，转录本的量明显增加。测序结果显示，此基因全长643bp，编码200个氨基酸。序列分析发现，此基因属于Ⅱ类内切几丁质酶基因。初步预测此基因的编码产物可能具有抗冻活性。 |
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其他语种文摘
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A new gene was isolated from cold-acclimated Saussurea involucrata Kar. et Kir. leaves by RT-PCR. Our experiment showed that this gene was induced by low temperature and there was an increase in the level of its transcript during cold acclimation. This gene is 643 bp in length and the predicted open reading frame contains 200 amino acids. Sequence comparisons between this gene and other plants＇ chitinase genes at the nucleotide level and at the amino acid level revealed that it encodes a class Ⅱ endochitinase. Moreover, the predicted product of this gene maybe has antifreeze activity. |
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来源
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植物生理学通讯
,2005,41(6):731-736 【核心库】
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关键词
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新疆雪莲
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冷诱导基因
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Ⅱ类内切几丁质酶类基因
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抗冻活性
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地址
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中国科学院新疆理化技术研究所, 新疆, 乌鲁木齐, 830011
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0412-0922 |
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学科
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植物学;农作物 |
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文献收藏号
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CSCD:2161070
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参考文献 共
7
共1页
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