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Generation and Characterization of C305, a Murine Neutralizing scFv Antibody That Can Inhibit BLyS Binding to Its Receptor BCMA

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文摘 B-lymphocyte stimulator (BLyS) is a member of the tumor necrosis factor (TNF) family and a key regulator of B cell response. Neutralizing single-chain fragment variable (scFv) antibody against BLyS binding to its receptor BCMA has the potential to play a prominent role in autoimmune disease therapy. A phage display scFv library constructed on pIII protein of M13 filamentous phage was screened using BLyS. After five rounds of panning, their binding activity was characterized by phage-ELISA. Nucleotide sequencing revealed that at least two different scFv gene fragments (C305 and D416) were obtained. The two different scFv gene fragments were expressed to obtain the soluble scFv antibodies, then the soluble scFv antibodies were characterized by means of competitive ELISA and in vitro neutralization assay. The results indicated that C305 is the neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA.
来源 Acta Biochimica et Biophysica Sinica ,2005,37(6):415-420 【核心库】
DOI 10.1111/j.1745-7270.2005.00059.x
关键词 phage display ; neutralizing ; scFv antibody ; scFv antibody
地址

1. College of Life Science and Technology, Jinan University, GZ, 广东, Guangzhou, 510632  

2. Shanghai Research Center of Biotechnology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200233

语种 英文
文献类型 研究性论文
ISSN 1672-9145
学科 生物化学
文献收藏号 CSCD:2147594

参考文献 共 18 共1页

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