文摘
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采用多倍体育种技术,对云南的野生热带兰花齿瓣石斛(Dendrobium devonianum)进行多倍体诱导试验.利用秋水仙素作为诱导剂,对二倍体试管苗进行诱导,在短期内培育出多倍体植株.用0.03%的秋水仙素处理齿瓣石斛丛生芽24 h,其诱导效果最好.观察结果显示:多倍体齿瓣石斛材料在形态、气孔直径、染色体数目上都有变化.DNA指纹图谱等方面都发生了显著的改变.多倍体齿瓣石斛试管苗出现了植株粗壮、叶片增厚等优良性状.此研究是多倍体育种技术在我国热带兰花育种中的一次成功尝试. |
其他语种文摘
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The polyploid breeding technique was used to induce polyploid of Dendrobium devonianum,an orchid occurring in the tropics of Yunnan with ornamental and medicinal values.The materials for polyploid breeding were in vitro seedlings of diploids.The polyploid plants could be induced in a short time with colchicines as mutagen.It resulted in the best inductive effect to use 0.03% colchicines to deal with the in vitro seedlings of diploids for 24 hours.The polyploid plants were different from those of diploids in morphology, slorna diameter, chromosome numbers, and !.)NA fingerprints. The in vitro seed-lings of polyploid plant showed advantageous characteristics such as thicker stems and leaves, [l is a suc-cessful case to apply polyploid breeding technique inlo the Iropical orchid breeding in China. |
来源
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云南植物研究
,2005,27(5):552-556 【核心库】
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关键词
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齿瓣石斛
;
多倍体
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地址
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1.
云南农业大学园林园艺学院, 云南, 昆明, 650201
2.
中国科学院昆明植物研究所, 云南, 昆明, 650204
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语种
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中文 |
文献类型
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研究性论文 |
ISSN
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0253-2700 |
学科
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植物学 |
基金
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国家科技部项目
;
中国科学院项目
;
国家自然科学基金
;
美国国家科学基金
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文献收藏号
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CSCD:2086023
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