滇牡丹遗传多样性的ISSR分析
Genetic diversity of Paeonia delavayi as revealed by ISSRs
查看参考文献34篇
|
文摘
|
应用ISSR标记对中国西南地区特有植物滇牡丹(Paeonia delavayi)的遗传多样性进行了研究.从100个引物中筛选出10个用于正式扩增,在取自16个自然居群和1个迁地保护居群的511个个体中,检测到92个多态位点.在居群水平上,多态位点百分率(PPB)为44.61%,Nei's基因多样性指数(H)和Shannon信息指数(I)分别为0.1657和0.2448.在物种水平上,多态位点百分率(PPB)为79.31%,Nei's基因多样性指数(H)和Shannon信息指数(I)分别为O.2947和0.4355.居群间的遗传分化系数(Gsr)达0.4349.结果表明:滇牡丹遗传多样性水平较高,居群间遗传分化较大.结合以前的研究结果,对滇牡丹的现状进行评估的结果显示,滇牡丹并不濒危. |
|
其他语种文摘
|
The genetic diversity of Paeonia delavayi, a species endemic to Southwest China, was ana-lyzed using inter-simple sequence repeat markers (ISSR). A total of 116 bands were amplified from 16 natural populations and one ex situ conserved population by 10 informative and reliable primers of 100 pre-screened primers. Of them, 92 ISSR loci were polymorphic. A relatively high level of intraspecific genetic diversity was revealed; PPB = 44, 61% , H =0.1657 and 7=0.2448 at population level; PPB - 79.31% , // = 0.2947 and / = 0.4355 at species level. The Gsr = 0.4349 indicated a high degree of genetic differentiation occurred among P. delavayi populations. The results of genetic diversity study, combined with field survey and previous investigations, suggest that P. delavayi is not endan-gered. |
|
来源
|
生物多样性
,2005,13(2):105-111 【核心库】
|
|
关键词
|
Paeonia delavayi
;
居群
;
居群遗传结构
;
遗传分化
|
|
地址
|
1.
中国科学院昆明植物研究所, 云南, 昆明, 650204
2.
中山大学生命科学学院, 广东, 广州, 510275
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1005-0094 |
|
学科
|
植物学;园艺 |
|
基金
|
云南省自然科学基金
|
|
文献收藏号
|
CSCD:1921549
|
参考文献 共
34
共2页
|
|
1.
Avise JC. Chapman & Hall.
Molecular Markers, Natural History and Evolu tion,1994
|
被引
1
次
|
|
|
|
|
2.
Ayres DR. Genetic diversity and structure of the narrow endemic Wyethia reticulate and its congener W.
American Journal of Botany,1999,86:344-353
|
被引
14
次
|
|
|
|
|
3.
Bawa KS. a genetic perspective.
Genetics and Conservation of Rare Plants,1991:62-71
|
被引
3
次
|
|
|
|
|
4.
方文培. Notes on Chinese paeonies.
植物分类学报,1958,7:297-323
|
被引
28
次
|
|
|
|
|
5.
冯国楣. Paeonia delavayi Franch.
Finet & Gagnep,1992
|
被引
1
次
|
|
|
|
|
6.
Fu LK. 中国植物红皮书)(ed.
中国植物红皮书:530-531
|
被引
1
次
|
|
|
|
|
7.
Frankel OH. The Conservation of Plant Biodiversity.
The Conservation of Plant Biodiversity,1995
|
被引
11
次
|
|
|
|
|
8.
葛颂. Plant Evolutionary Biology (植物进化生物学)(eds Chen JK (陈家宽).
植物进化生物学,1994:153-208
|
被引
18
次
|
|
|
|
|
9.
葛颂. Application of DNA molecular marker inconservation biology.
系统与进化植物学中的分子标记,2001:140-149
|
被引
5
次
|
|
|
|
|
10.
Ge S. Allozyme variation in Ophiopogon xylorrhizus.
Conservation Biology,1997,11:562-565
|
被引
11
次
|
|
|
|
|
11.
龚洵. 黄牡丹七个居群的细胞学研究.
云南植物研究,1991,13:402-410
|
被引
13
次
|
|
|
|
|
12.
龚洵. 滇牡丹的多样性和现状评估.
西北植物学报,2003,23:218-223
|
被引
21
次
|
|
|
|
|
13.
龚洵. 黄牡丹八个居群的Giemsa C-带比较研究.
云南植物研究,1999,21:477-482
|
被引
7
次
|
|
|
|
|
14.
Hamrick JL. Conservation genetics of endemic plant species.
Conservation Genetics,1996:281-304
|
被引
10
次
|
|
|
|
|
15.
Hamrick JL. Factors influencing levels of genetic diversity in woody plant species.
New Forests,1992,6:95-124
|
被引
161
次
|
|
|
|
|
16.
Helenurm K. High levels of genetic polymorphism in the insular endemic herb Jepsonia malvifolia.
Journal of Heredity,2001,92:427-432
|
被引
6
次
|
|
|
|
|
17.
Hickey RJ. Genetic variation in running buffals clover Trifolium soloniferum.
Conservation Biology,1991,5:309-316
|
被引
8
次
|
|
|
|
|
18.
Hogbin PM. Evaluation of the contribution of genetic research to the management of the endangered plant Zieria prostrata.
Conservation Biology,1999,13:514-522
|
被引
64
次
|
|
|
|
|
19.
Hong D Y. Flora of China.
Flora of China.6,2001
|
被引
1
次
|
|
|
|
|
20.
Hong DY. Taxonomy of the Paeonia delavayi complex (Paeoniaceae).
Annals of the Missouri Botanical Gardens,1998,85:554-564
|
被引
31
次
|
|
|
|
|
了解气象卫星更多信息，请访问