利用微量热法研究Cd2+和Cu2+对嗜热四膜虫(Tetrahymena thermophila)的毒性效应
TOXIC EFFECTS OF Cd2+ AND Cu2+ ON TETRAHYMENA THERMOPHILA BY MICROCALORIMETRY
查看参考文献16篇
文摘
|
利用微量热法研究Cd2+和Cu2+对嗜热四膜虫BF5 (Tetrahymena thermophila BF5)生长代谢毒性效应,结果表明: ①低浓度的Cd2 + (0~0.4 mg L-1)和Cu2+(0~10 mg L-1)对四膜虫的生长有促进作用,而高浓度的Cd2 +(0.8~3.2 mg L-1)和Cu2+(20~200 mg L-1)则产生抑制作用;②Cd2+和Cu2+的半抑制浓度IC50分别为2.04 mg/L和155.5 mg/L;③联合毒性为协同作用;④微量热法所得Cd2+和Cu2+单独作用时的IC10分别为0.96 mg/L和19.9 mg/L,高于种类损伤法结果;⑤微量热法所得Cd2+和Cu2+单独作用时的IC10均高于II级饮用水水质标准.而Cd2+和Cu2+间的协同作用使Cd2+的IC10降低为0.31 mg/L,高于水质标准;Cu2+的IC10降低为1.16 mg/L,与水质标准相近.总之,微量热法不仅是一种快速评价污染物毒性效应的新方法,而且可用于相关的环境毒理学理论研究. |
其他语种文摘
|
The toxic effects of Cd2+ and Cu2+ along and combined with Tetrahymena ihcrmophila BF5 were studied by microcal-orimetry. The results showed that;;?low concentrations of Cd2+ (0 -0.4 mg L ) and Cu"+ (0-10 mg L ) promoted the growth of T. thermophila BF5 , while high concentrations of Cd22+(0. 8 -3. 2 nig L~' ) and Cu2t (20 -200 mg L2+ ) inhibited ils growth;(2)tli(J half inhibition concentrations 1C5(I of Cd2 * and Cu3' were 2.04 mg/L and 155.5 mg/L, respectively ;C|)llit' joint toxicity of them was synergism ;(4)lhe IC,,, of Cd2+ and Cu2 ' obtained by niicrofulorimetry were 0. 96 mg/L and 19. 9 mg/ L, ami they were both higher than ECH) by laxonotnic loss;(5jtlie ICIO of Cd2+ and Cu2+ obtained by ink'roealorinietry were both higher than water quality standards, but synergism of Cd2+and Cu2+ made their IC,,> lower. The ICIU of Cd2+ became 0. 31 mg/ L, which was higher than drinking water quality standards II, and that of Cu2+ was 1. 16 mg/L, which was close In waler quali-ty standards. In a word, microcalorimelry was not only a new fast response technique lor evaluating ihe toxic effects ol pollu-tants, but also useful to the corresponding environmental lexicological theoretic studies. |
来源
|
应用与环境生物学报
,2004,10(6):745-749 【核心库】
|
关键词
|
微量热
;
Cd2+
;
Cu2+
;
嗜热四膜虫
;
联合毒性
;
种类损伤法
|
地址
|
1.
中国科学院水生生物研究所, 武汉, 430072
2.
武汉大学化学与分子科学学院, 武汉, 430072
|
语种
|
中文 |
文献类型
|
研究性论文 |
ISSN
|
1006-687X |
学科
|
环境科学基础理论 |
基金
|
国家自然科学基金项目
;
中国科学院知识创新工程重要方向项目
|
文献收藏号
|
CSCD:1809410
|
参考文献 共
16
共1页
|
1.
许木启. Ecolo.
动物学集刊,1995,12:346-350
|
被引
2
次
|
|
|
|
2.
Gustafsson L. 1994 and all thcology in acalorimeter.
Thermochim Acta,1995,251:69-70
|
被引
2
次
|
|
|
|
3.
Yan C N. Thermochemical studies of the toxic actions of heavy metal ions on Rhizopus nigricans.
Chemospere,1999,38(4):891-898
|
被引
6
次
|
|
|
|
4.
Li X. Microcalorimetric study of Staphylococcus aureus growth affected by selenium compounds.
Thermochim Acta,2002,387:57-61
|
被引
17
次
|
|
|
|
5.
Sauvant M P. Tetrahymeological studies.
Chemosphere,1999,38(7):1631-1669
|
被引
10
次
|
|
|
|
6.
Beermann K. A calorimetric method for the rapid evaluation of toxic substances using Tetrahymena pyriformis.
Thermochim Acta,1999,337:65-69
|
被引
5
次
|
|
|
|
7.
Yao J. Microcalorimetric investigation of the effect of manganous (Ⅱ) on the growth of Tetrahymena shanghaiensis S1 99.
Biol Trace Element Res,2003,92:71-82
|
被引
2
次
|
|
|
|
8.
Ingemar Wadso. Isothermal microcalorimetry in applied biology.
Thermochim Acta,2000,394:305-311
|
被引
1
次
|
|
|
|
9.
沈韫芬. Evaluating ecotoxicolo.
第七届海峡两岸环境保护学术研讨会下卷,2001:33-41
|
被引
1
次
|
|
|
|
10.
Xie C L. Microcalorimetric study of bacterial growth.
Thermochim Acta,1988,123:33-41
|
被引
59
次
|
|
|
|
11.
任继平. A.
动物营养学报,2003,15(1):1-6
|
被引
31
次
|
|
|
|
12.
Wakatsukl T. Respiratory inhibition by copper intake in Tetrahymena pyriformis GL.
J Ferment Technol,1986,64(2):119-127
|
被引
1
次
|
|
|
|
13.
Dunlop S. Detoxication of Zinc and Cadmium by the Freshwater Protozoan Tetrahymena pyriformis.
Environ Res,1981,24(2):264-274
|
被引
1
次
|
|
|
|
14.
Sauvant M P. Toxicity assessment of 16 inorganic environment pollutants by six bioassays.
Ecotoxicolo & Environ Safety,1997,37(40):131-140
|
被引
3
次
|
|
|
|
15.
杨再福. 重金属铜、镉对蝌蚪毒性的研究.
中国生态农业学报,2003,11(1):102-103
|
被引
19
次
|
|
|
|
16.
王悠. 蒽与有机磷农药对海洋微藻的联合毒性.
海洋科学,2000,24(4):5-6
|
被引
5
次
|
|
|
|
|