HPLC法测定獐牙菜及其近缘植物中4种有效成分的含量
Simultaneous Determination of Four Effective Components in Swertia and Its Related Plants by HPLC
查看参考文献8篇
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文摘
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目的:建立反相高效液相色谱法同时测定獐牙菜及其近缘植物中番木鳖酸、獐牙菜苦苷、龙胆苦苷、芒果苷的含量。方法:采用ZORBAX SB—C18(250 mm×4.6mm,5μm)色谱柱,以流动相甲醇和水(含0.04%磷酸)的比例在0-24 min内由22:78至38:62线性梯度洗脱,流速1 mL·min～1,检测波长254 nm,柱温30℃。结果:4种成分均达到基线分离,番木鳖酸、獐牙菜苦苷、龙胆苦苷、芒果苷的线性范围分别为0.05—6.25μg(r=0.9999),0.0095—2.9 μg(r=0.9998),0.0486—2.56μg(r=0.9999),0.0056—2.8μg(r=0.9998);回收率为102%(RSD=4.4%),97.7%(RSD=4.3%),99.5%(RSD=3.5%),103%(RSD=1.1%)。结论:方法测定快速,结果准确、可靠。 |
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其他语种文摘
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Objective: To establish a quantitative method of simultaneously determination of loganic acid, swer-tiamarin, gentiopicroside and mangiferin in Swertia and its related plants by RP - HPLC. Method: The sample were separated on the column of Zorbax SB - C_(18) (250 mm * 4. 6 mm, 5 μm) which eluted with methanol and water (0, 04% phosphoric acid). The ratio of methanol and water increased from 22:78 to 38:62 in 24 min with detected wavelength at 254 run, flow rate at 1 mL·min~(-1), column temperature at 30 ℃. Results: Four compounds were base - isolated. The linear ranges of loganic acid, swertiamarin, gentiopicroside and mangiferin were 0. 05-6. 25 μg(r = 0. 9999), 0. 0095 -2. 9 μg(r =0. 9998), 0. 0486 -2. 56 μg(r =0. 9999), 0. 0056 -2. 8 μg(r =0. 9998) respectively; The average recoveries were 102% (RSD =4. 4%), 97. 7% (RSD =4. 3%), 99. 5% (RSD =3. 5%), 103% (RSD =1.1%)respectively. Conclusions: The method is rapid and precise. |
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来源
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药物分析杂志
,2004,24(5):502-505 【核心库】
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关键词
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反相高效液相色谱
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番木鳖酸
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獐牙菜苦苷
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龙胆苦苷
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芒果苷
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獐牙菜
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龙胆科
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地址
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1.
青海民族学院化学系, 西宁, 810007
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中国科学院西北高原生物研究所, 西宁, 810001
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0254-1793 |
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学科
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药学 |
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基金
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青海民族学院基金
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文献收藏号
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CSCD:1807582
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