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两种缘毛类纤毛虫胞质Hsp70基因序列的克隆及其系统发育分析
Phylogenetic Analysis of Peritrichs Inferred from Cytosolic Hsp70 Gene Partial Sequences

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傅诚杰 1   缪炜 1   沈韫芬 2   万明亮 2  
文摘 克隆得到2种缘毛类纤毛虫--钟形钟虫(Vorticella campanula)和螅状独缩虫(Carchesium polypinum)的胞质Hsp70基因部分序列,长度均为438 bp,编码146个氨基酸.以细菌为外类群,利用最大似然法和邻接法构建包括其他5种纤毛虫在内的共26个物种的Hsp70基因氨基酸序列系统发育树,其拓扑结构显示:V.campanula和C.polypinum聚在一起,并与另2种寡膜纲的嗜热四膜虫(Tetrahymena thermophila)及草履虫(Paramecium tetraurelia)聚为姊妹枝,提示了缘毛类纤毛虫为单系,且隶属于寡膜纲的系统发育地位.
其他语种文摘 Cytosolic Hsp70 genes of two peritrichous ciliates (Vorticella campanula & Carchesium polypinum) were partially sequenced and the lengths are 438 bp, both encoding 146 amino acids. Using bacteria as the outgroup, the phylogenetic trees were constructed based on cytosolic Hsp70 amino acid sequences by maximum-likelihood (ML) and neighbor-joining (NJ) methods. The topology that V. campanula clustered with C.polypinum, and then formed a sister clade with another two species in Oligohymenophorea (Tetrahymenu thermophila & Paramecium tetraurelia), suggested the nophyly of the peritrichs and their phylogenetic placement in the class Oligohymenophorea.
来源 动物学研究 ,2004,25(4):298-303 【核心库】
关键词 缘毛类纤毛虫 ; 胞质Hsp70基因 ; 系统发育
地址

1. 中国科学院东湖湖泊生态系统试验站, 湖北, 武汉, 430072  

2. 中国科学院水生生物研究所, 湖北, 武汉, 430072

语种 中文
文献类型 研究性论文
ISSN 0254-5853
学科 动物学
基金 中国科学院知识创新工程项目 ;  中国科学院东湖湖泊生态系统试验站开放研究课题基金
文献收藏号 CSCD:1795085

参考文献 共 31 共2页

1.  Bardele CF. A comparative freeze-fracture study[J]. BioSystems,1981,14:403-421 被引 3    
2.  Bernhard DD. Evolution of histone H4 and H3 gene in different ciliate lineages[J]. J.Mol.Evol.,1998,46:344-354 被引 6    
3.  Budin K. New insights into the phylogeny of eukaryotes based on ciliate Hsp70 sequences[J]. Mol.Biol.Evol.,1998,15:943-956 被引 7    
4.  Corliss JO. The value of ontogenetic data in reconstructing protozoan phylogenies[J]. Trans.Amer.Micros.Soc.,1968,87:1-20 被引 2    
5.  Corliss JO. Historical analysis of past efforts and a newly proposed phylogenetic scheme of classification for the protistan phylum Ciliophora[J]. Syst.Zool.,1974,23:91-138 被引 3    
6.  Corliss JO. Characterization. The Ciliated Protozoa:Characterization,Classification and Guide to the Literature.2nd ed.,1979 被引 1    
7.  Fast NM. Re-examining alveolate evolution using multiple protein molecular phylogenies[J]. J.Eukaryot.Microbiol.,2002,49:30-37 被引 5    
8.  Felsenstein J. An approach using the bootstrap[J]. Evolution,1985,39:783-791 被引 729    
9.  Finley HE. The peritrichs. Trans.Amer.Micros.Soc.,1974,93:307-313 被引 1    
10.  Galtier N. Inferring phylogenies from DNA sequences of unequal base compositions[J]. Proc.Natl.Acad.Sci.USA,1995,92:11317-11321 被引 4    
11.  Germot A. A case study based on the Hsp70 family[J]. J.Eukaryot.Microbiol.,1999,46:116-124 被引 1    
12.  Greenwood SJ. Phylogenetic relationships within the class Oligohymenophorea. J.Mol.Evol.,1991,33:163-174 被引 6    
13.  Gupta RS. Evolution of Hsp70 gene and its implications regarding relationships between archaebacteria. J.Mol.Evol.,1993,37:573-582 被引 4    
14.  Hasegawa M. Ribosomal RNA trees misleading[J]. Nature,1993,361:23 被引 9    
15.  Hasegawa M. Dating of the human-ape splitting by a molecular clock of mitochondrial DNA[J]. J.Mol.Evol.,1985,21:160-174 被引 43    
16.  Hoffman DC. Phylogenetic relationships among hypotrichous ciliates determined with the macronuclear gene encoding the large. J.Mol.Evol.,1997,45:301-310 被引 6    
17.  Leeuwenhoek AV. More observations from Mr. Phil.Trans.Roy.Soc.,9:178-182 被引 1    
18.  Leipe DD. Small subunit ribosomal RNA+ of Hexamita inflate and the quest for the first branch in the eukaryotic tree[J]. Mol.Biochem.Parasitol.,1993,59:41-48 被引 6    
19.  Lom J. Observations on the ultrastructure of the buccal apparatus in thigmotrich ciliates and their bearing on thigmotrich-peritrich affinities[J]. J.Protozool.,1968,15:824-840 被引 1    
20.  Miao W. Phylogenetic relationships of the subclass peritrichia (Oligohymenophorea. J.Eukaryot.Microbiol.,2001,48:583-587 被引 9    
引证文献 2

1 冯立芳 不同纬度螅状独缩虫耐热能力及Hsp70 mRNA表达水平的比较 动物学报,2008,54(3):525-530
被引 2

2 王勇 qRT-PCR检测柞蚕中肠热休克蛋白基因ApHSC70对微孢子虫入侵的响应 蚕业科学,2015,41(2):300-307
被引 4

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