两种缘毛类纤毛虫胞质Hsp70基因序列的克隆及其系统发育分析
Phylogenetic Analysis of Peritrichs Inferred from Cytosolic Hsp70 Gene Partial Sequences
查看参考文献31篇
文摘
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克隆得到2种缘毛类纤毛虫--钟形钟虫(Vorticella campanula)和螅状独缩虫(Carchesium polypinum)的胞质Hsp70基因部分序列,长度均为438 bp,编码146个氨基酸.以细菌为外类群,利用最大似然法和邻接法构建包括其他5种纤毛虫在内的共26个物种的Hsp70基因氨基酸序列系统发育树,其拓扑结构显示:V.campanula和C.polypinum聚在一起,并与另2种寡膜纲的嗜热四膜虫(Tetrahymena thermophila)及草履虫(Paramecium tetraurelia)聚为姊妹枝,提示了缘毛类纤毛虫为单系,且隶属于寡膜纲的系统发育地位. |
其他语种文摘
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Cytosolic Hsp70 genes of two peritrichous ciliates (Vorticella campanula & Carchesium polypinum) were partially sequenced and the lengths are 438 bp, both encoding 146 amino acids. Using bacteria as the outgroup, the phylogenetic trees were constructed based on cytosolic Hsp70 amino acid sequences by maximum-likelihood (ML) and neighbor-joining (NJ) methods. The topology that V. campanula clustered with C.polypinum, and then formed a sister clade with another two species in Oligohymenophorea (Tetrahymenu thermophila & Paramecium tetraurelia), suggested the nophyly of the peritrichs and their phylogenetic placement in the class Oligohymenophorea. |
来源
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动物学研究
,2004,25(4):298-303 【核心库】
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关键词
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缘毛类纤毛虫
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胞质Hsp70基因
;
系统发育
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地址
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1.
中国科学院东湖湖泊生态系统试验站, 湖北, 武汉, 430072
2.
中国科学院水生生物研究所, 湖北, 武汉, 430072
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语种
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中文 |
文献类型
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研究性论文 |
ISSN
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0254-5853 |
学科
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动物学 |
基金
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中国科学院知识创新工程项目
;
中国科学院东湖湖泊生态系统试验站开放研究课题基金
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文献收藏号
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CSCD:1795085
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