昆明山海棠提取物对血管平滑肌细胞增殖及凋亡的影响
Effect of Extract from Tripterygium Hypoglaucum on Proliferation and Apoptosis of Vascular Smooth Muscle Cells
查看参考文献9篇
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文摘
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目的探讨昆明山海棠提取物(THW 4)对离体血管平滑肌细胞(VSMC)增殖及凋亡的影响.方法培养家兔主动脉平滑肌细胞,加入不同浓度的 THW 4进行处理,用MTT法测定细胞生长曲线,以Annexin V FITC/PI双标记、透射电镜、原位末端标记法检测细胞凋亡.结果 THW 4可以明显抑制VSMC增殖,呈剂量和时间依赖性,作用48h的半有效抑制浓度(IC 50 )为15.6μg/L;VSMC在含10～100μg/L THW 4的培养液中孵育56h,可检测到细胞早期凋亡,且凋亡率随THW 4浓度增加而升高;延长孵育时间至72h,透射电镜下可观察到典型的凋亡细胞形态学改变,原位末端标记法显示DNA片段化增多,有核碎裂、固缩等改变;细胞周期时相分布显示THW 4主要阻滞VSMC于G2/M期.结论 THW 4能有效抑制体外培养的VSMC增殖及诱导其凋亡,提示THW 4可能会作为防治血管成形术后再狭窄的候选药物. |
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其他语种文摘
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Objective To study the effect of THW-4, an extract from Trypterygium hypoglaucum on proliferation and apoptosis of vascular smooth muscle cells (VSMC). Methods VSMC derived from rabbit aorta were cultured in vitro and different concentrations of THW-4 were added in experimental groups. Cell proliferation was evaluated by MTT and apoptosis by transmission electron microscopy (TEM), TUNEL assay and Annexin V-FITC/PI double labelled assay. Results The inhibitory effects of THW-4 on proliferation of VSMC displayed dose-time dependently, with the IC50 value of 15.6 jxg/L at 48 hrs. Incubated with THW-4 (10 - 100 fig/L) for 56 hrs, VSMC mainly appeared early stage apoptosis and the percentage of apoptosis was found to raise along with the increase of the THW-4 concentration. Typical images of apoptosis could be observed under TEM and TUNEL assay showed increase of DNA segments with karyorrhexis and pyknosis after THW-4 treatment for 72 hrs. Analysis of cell cycle indicated the THW-4 mainly lead to the blockage of VSMC in G2/M stage. Conclusion THW-4 could inhibit the proliferation and induce the apoptosis of VSMC in vitro, suggesting that THW-4 is a potential agent for prevention of restenosis following angioplasty. |
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来源
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中国中西医结合杂志
,2004,24(9):827-830 【核心库】
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关键词
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昆明山海棠
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提取物
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血管平滑肌细胞
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增殖
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凋亡
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地址
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1.
北京大学第一医院心内科, 北京, 100034
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北京大学第三医院心内科
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中国科学院昆明植物研究所
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1003-5370 |
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学科
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中国医学 |
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文献收藏号
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CSCD:1698622
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