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文摘
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目的观察千金子甲醇提取物体内、外抗肿瘤活性及其量效关系.方法体外药效试验用MTT法,体内用小鼠移植性肿瘤,观察千金子甲醇提取物的抑瘤活性. 结果千金子甲醇提取物体外对人宫颈癌细胞(Hela)、人红白血病细胞( K562)、人单核细胞性白血病细胞(U937)、人急性淋巴细胞性白血病细胞( HL60) 和人肝癌细胞(HepG2)的IC50值分别为15.5μg/ml、13.1μg/ml、10.5μg/ml、17.5μg/ml、29.6μg/ml;体内对小鼠移植性肿瘤细胞株也显示出较显著抑制作用. 结论千金子甲醇提取物体外对Hela、K562、U937、HL60 、HepG2 有明显的细胞毒活性,体内对小鼠肉瘤180(S180)和艾氏腹水癌(EAC)也显示出较好的抑制作用. |
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其他语种文摘
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Objective To study the antitumor activity of methanolic extractive from Euphorbia Lathyris L. both in vitro and in vivo, and further evaluate its does-effect relationship. Methods In vitro and in vivo study on the pharmacological activity of methanolic extractive from Euphorbia Lathyris L. were performed by using MTT assay and mice transplant tumor model respectively. Results IC_(50) numerical value of Hela cells, K_(562) cells, U_(937) cells, HL_(60) cells and HepG_2 cells were 15.5μg/ml, 13.1μg/ml, 10.5μg/ml, 17.5μg/ml, 29.6μg/ml in vitro respectively, in addition methanolic extractive from Euphorbia Lathyris L. demonstrated antitumor activity in vivo. Conclusion Methanolic extractive from Euphorbia lathyris L. has not only obvious inhibition effect on the human tumor cell strains such as Hela cells.,U_(937) cells in vitro, but also demonstrated antitumor effect on mice transplant tumors such as Siro ,EAC in vivo. |
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来源
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肿瘤防治研究
,2004,31(9):556-558 【扩展库】
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关键词
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千金子甲醇提取物
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MTT法
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人癌细胞株
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肉瘤180
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艾氏腹水癌
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抗肿瘤
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地址
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1.
华中科技大学同济医学院附属同济医院中西医结合研究所, 武汉, 430030
2.
华中科技大学同济医学院附属梨园医院老年医学研究所
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-8578 |
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学科
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肿瘤学 |
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文献收藏号
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CSCD:1655698
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