用紫外可见分光光度法测定共轭亚油酸的含量
Quantitation of Coniugated Linoleic Acid by UV Spectrophotometry
查看参考文献14篇
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文摘
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采用UV-9100型紫外可见分光光度计,测定了共轭亚油酸、共轭亚油酸甲酯、共轭亚油酸三甘酯在不同浓度时在200～300 nm的吸光度,绘制出样品在不同浓度时的吸光度曲线,找出适宜的浓度范围;用Microcal Software Origin Version 4.0做回归分析,由线性回归给出样品浓度和吸光度的线性关系;利用PE Autosystem XL-TurboMass测得样品中共轭亚油酸乙酯的准确浓度,结合回归系数给出了测定样品中共轭亚油酸含量的经验公式. |
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其他语种文摘
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Through absorbance measurement of conjugated linoleic acid, methyl ester of conjugated lin-oleic acid and conjugated linoleic acid triglycide from 210-~300 nm by UV-9100 spectrophotometer, ab-sorbancy curve of measured samples at different concentration is plotted. In the appropriate concentra-tion range of samples, linear relationship of absorbancy and samples' concentration is presented by line-ar regression with Microcal Software Origin Version 4. 0 system. The accurate concentration of conj u-gated linoleic acid is obtained with PE Autosystem XL-TruboMass. Combined with regression constant data , emprical formula for the quantitation of conjugated linoleic acid by UV spectrophotometer is giv-en. |
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来源
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中国科学院研究生院学报
,2002,19(4):436-442 【核心库】
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关键词
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紫外可见分光光度法
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共轭亚油酸
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十八碳二烯酸
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地址
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1.
中国科学院研究生院, 北京, 100039
2.
中国科学院新疆理化技术研究所, 乌鲁木齐, 830011
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1002-1175 |
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学科
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化学工业 |
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基金
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中国科学院西部之光人才培养计划
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文献收藏号
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CSCD:1366507
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参考文献 共
14
共1页
|
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1.
Iversen S A. Identification of a diene conjugated component of human lipid as octadeca-9.
FEBSLett,1984,171(2):320-324
|
被引
5
次
|
|
|
|
|
2.
Situnayake R D. Use second-derivative spectroscopy to quantify conjugated dienes.
Ann Clin Biochem,1990,27(3):258-266
|
被引
3
次
|
|
|
|
|
3.
曹栋. 脱水蓖麻油的研究.
中国油脂,1999,24(4):55-56
|
被引
3
次
|
|
|
|
|
4.
Homing E C. Quantitative Analysis of Fatty Acids by Gas-Liquid Chromatography.
J Lipid Res,1964,5:20-27
|
被引
1
次
|
|
|
|
|
5.
Wemer A S. effects of cheese cultures.
J Agric Food Chem,1992,40:1817-1821
|
被引
1
次
|
|
|
|
|
6.
Fulk W K. Production of an artifact during methanolysis of lipids by boron trifluoride-methanol.
J Lipid Res,1970,11:276-277
|
被引
3
次
|
|
|
|
|
7.
Dawidowicz E A. Artifacts produced by boron trifluoride rnethanolysis of a synthetic lectin containing cyclopropane fatty acids(1-2-dihydrosterculoyl-3-snphosphatidyl choline).
J Lipid Res,1971,12:636-637
|
被引
1
次
|
|
|
|
|
8.
Luddy F E. Direct conversion of lipid compotents to their fatty acid methyl esters.
J Am Oil Chem Soc,1960,37:447-451
|
被引
1
次
|
|
|
|
|
9.
Kramer J K G. Evaluating acid and base catalysts in the methyation of milk and rumen fatty acids with special emphasis on conjugated dienes and total trans fatty acids.
Lipids,1997,32:1219-1228
|
被引
9
次
|
|
|
|
|
10.
Lorette N B. Use of acetone dimethyl acetal in preparation of methyl ester.
J Org Chem,1959,24:261-262
|
被引
1
次
|
|
|
|
|
11.
. Nalur Chandrasekaran Shantha.
J of AOAC International,1993,76:644-649
|
被引
1
次
|
|
|
|
|
12.
Masao Yamasaki. A recommended esterification method for gas chromatographic measurement of conjugated linoleic acid.
J Am Oil Chem Soc,1999,76:933-938
|
被引
8
次
|
|
|
|
|
13.
徐生庚(译).
油脂化学与工艺学(第5版第2卷),2001:71
|
被引
1
次
|
|
|
|
|
14.
Rossell J B. Rancidity in foods.
Rancidity in foods.,1987:21-45
|
被引
1
次
|
|
|
|
|
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