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松针褐斑病菌毒素活性的生物检测
Studies on the Bioassay of Toxins fromLecanosticta acicola Causing Brown spot Needle Blight of Pines

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文摘 松针褐斑病菌毒素不仅能伤害湿地松、火炬松等寄主植物的幼苗、针叶、根及愈伤组织,对非寄主植物水花生、紫茎泽兰的叶片也有伤害作用。在试验的几种生测材料中,湿地松愈伤组织对毒素伤害最敏感,湿地松幼苗、幼针叶,火炬松幼针叶和紫茎泽兰叶片次之,湿地松幼根、老针叶,火炬松老针叶,水花生和小麦叶片最不敏感。根据生测材料的敏感程度及表现症状的特异性分析可知,用湿地松、火炬松幼苗和幼针叶作生测材料较好。比较针刺法、浸渍法和涂抹法等几种生测方法表明,用针法接种毒素效果最好,其反应速度快,用量少,症状明显。因此建议在松针褐斑病菌毒素的生物检测中,以湿地松或火炬松幼苗或 针叶作为材料,采用针刺法。
其他语种文摘 Brown spot needle blight of pines is one of important pine diseases, which is caused by Lecanosticta acicola. It is confirmed that the fungi can produce toxic substances in its culture filtrate. The toxic substances ware called LA-toxins. LA-toxins could not only poison pine needles also the leaves of weeds. Various bioassay materials were treated with crude LA-toxin to select sensitive material for the future study. The results indicated that seedlings and young needles of sensitive host (slash pine, lobollolly pine) were selected as bioassay materials for their sensitivities and reaction of the typical necrotic and yellow bands on needles. Such symptoms were similar to the symptoms of natural infection in the field and were different from other injuries. Old needles of pines and weed leaves were not sensitive to LA-toxins. Compared with other two bioassay methods (soaking and smearing), the acupuncturing bioassay is the most suitable method because it just needs less toxin and can cause the evident symptom rapidly.
来源 南京林业大学学报. 自然科学版 ,2002,26(1):27-31 【核心库】
关键词 松树病害 ; 松针褐斑病菌 ; 真菌毒素 ; 松针褐斑病
地址

南京林业大学, 江苏, 南京, 210037

语种 中文
文献类型 研究性论文
ISSN 1000-2006
学科 林业
基金 国家自然科学基金
文献收藏号 CSCD:1041495

参考文献 共 6 共1页

1.  Hawes M C. Technique for using isolated corn root loop cells in a simple, quantative bioassay for the pathotoxin produced by Helminthosporium maydis Race T. Phytopathology,1983,73:1184-1187 被引 3    
2.  叶建仁. 松针褐斑病菌的产毒培养和毒素粗提方法. 南京林业大学学报,2001,25(5):6-10 被引 6    
3.  祁高富. 松针褐斑病菌毒素的确定及其基本性质研究. 南京林业大学学报,1999,23(4):17-21 被引 12    
4.  叶建仁. 松针褐斑病菌毒素的专化性研究. 南京林业大学学报,1999,23(6):1-4 被引 9    
5.  叶建仁. 松针褐斑病菌毒素对寄主细胞质膜伤害机理的研究. 林业科学,2000,36(2):82-86 被引 5    
6.  杨斌. 松针褐斑病菌毒素LA-Ⅱ的分离纯化及其化学结构. 南京林业大学学报,2001,25(3):21-25 被引 8    
引证文献 1

1 韩珊 板栗愈伤组织对栗疫菌Cp-毒素的抗性响应 南京林业大学学报. 自然科学版,2015,39(5):1-6
被引 1

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