栉孔扇贝16S rRNA基因片段序列的多态性研究
Sequence polymorphism of mitochondrial 16S rRNA gene fragment in scallop chlamys farreri
查看参考文献15篇
|
文摘
|
采用PCR技术扩增了栉孔扇贝（Chlamys farreri）线粒体DNA的16S rRNA基因片段，PCR产物经T载体连接之后进行了克隆、测序，得到634bp的核苷酸序列；分析了该片段的大连、烟台、青岛、朝鲜4个自然群体31个个体的核苷酸序列多态性，共检测到26个多态性核苷酸位点、18种单倍型。结果表明，4个群体中以朝鲜群体遗传多样性最高，其次为烟台、青岛群体，大连群体最低；不同自然分布区的栉孔扇贝未出现明显的遗传分化。 |
|
其他语种文摘
|
DNA sequencing is very useful for the genetic diversity analysis at molecular level. Mitochondrial 16S rRNA gene fragment of Scallop Chlamys farreri was amplified via polymerase chain reaction (PCR), the PCR products were ligated into T-vector, cloned and sequenced for 31 individuals, 3 populations of China (8 for Dalian and Qingdao each, and 7 for Yantai) and one population of Republic of Korea. 634 base-pair nucleotide sequences were examined and analyzed for genetic polymorphism. Resultant sequences data analysis showed that all 31 sequences were grouped into 18 haplotypes and there were 26 variable nucleotide positions in this gene fragment, which included transitions and transvertions, and no insertion/deletion were observed. The genetic diversity indexes including S (polymorphic sites), P (nucleotide diversity) and K (average number of nucleotide difference) were calculated and the results indicated that Korea population showed more variation than others and more differences to others, followed by Yantai and Qingdao population, and Dalian population had the least sequence variation. Parameters of genetic variation among the four populations suggested that no significant genetic difference was observed. Our data provided baseline information at DNA level on the genetic variation in wild Scallop populations and may be useful for future management and utilization of the resource. |
|
来源
|
海洋与湖沼
,2002,33(5):477-483 【核心库】
|
|
关键词
|
栉孔扇贝
;
线粒体DNA
;
16S rRNA基因
;
DNA序列测定
|
|
地址
|
1.
青岛海洋大学, 教育部海水养殖重点实验室, 青岛, 266003
2.
中国科学院海洋研究所, 青岛, 266071
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
0029-814X |
|
学科
|
动物学 |
|
基金
|
国家973计划
;
国家自然科学基金
|
|
文献收藏号
|
CSCD:1014680
|
参考文献 共
15
共1页
|
|
1.
王运涛. 栉孔扇贝大规模死亡的原因探讨.
海洋与湖沼,1999,30(6):770-774
|
被引
47
次
|
|
|
|
|
2.
李红蕾. 栉孔扇贝不同种群的遗传结构及其杂种优势.
海洋与湖沼,2002,33(2):188-195
|
被引
20
次
|
|
|
|
|
3.
邱高峰. 中国对虾16SrRNA基因序列多态性的研究.
动物学研究,2000,21(1):35-40
|
被引
28
次
|
|
|
|
|
4.
张亚平. 从DNA序列到物种树.
动物学研究,1996,17(3):247-252
|
被引
58
次
|
|
|
|
|
5.
陈再忠. 栉孔扇贝三个自然群体遗传变异初步研究.
中国贝类学论文集（IX）,2001:54-58
|
被引
1
次
|
|
|
|
|
6.
Boulding E G. Genetic variation in bottlenecked and two wild populations of the Japanese scallop (Patinopecten yessoensis): empirical parameter estimates from coding regions of mitochondrial DNA.
Can J Fish Aquat Sci,1993,50:1147-1157
|
被引
14
次
|
|
|
|
|
7.
Canapa A. Molecular data from the 16S rRNA gene for the phylogeny of Pectinidae (Mollusca: Bivalvia).
J Mol Evol,2000,50:93-97
|
被引
23
次
|
|
|
|
|
8.
Guo X M. Molluscan aquaculture in China.
J Shell Res,1999,18(1):19-31
|
被引
23
次
|
|
|
|
|
9.
Kitaura J. Genetic variation in populations of the diamond-shaped squid Thysanoteuthis rhombus as examined by mitochondrial DNA sequence analysis.
Fisheries Science,1998,64(4):538-542
|
被引
4
次
|
|
|
|
|
10.
Machado E G. Mitochondrial 16S rRNA gene of two species of shrimps: sequence variability and secondary structure.
Crustaxeana,1993,65(3):279-286
|
被引
4
次
|
|
|
|
|
11.
Mulvey M. Application of molecullar genetic markers to conservation of freshwater bivalves.
J Shell Res,1998,17(5):1395-1405
|
被引
6
次
|
|
|
|
|
12.
Palumbi S R. Large mitochondrial DNA differences between morphologically similar penaeid shrimp.
Mol Mar Biol Biotech,1991,1(1):27-34
|
被引
10
次
|
|
|
|
|
13.
Patwary M U. The use of random amplified polymorphic DNA markers in genetic studies of the sea scallop Placopecten magellanicus (Gmelin, 1791).
J Shell Res,1994,13(2):547-553
|
被引
8
次
|
|
|
|
|
14.
Suneetha K B. Analysis of mitochondrial DNA sequence from two Maurolicus taxa: evidence for separate species?.
J Fish Biol,2000,57:1605-1609
|
被引
7
次
|
|
|
|
|
15.
Wilbur A. Mitochondrial genotype variation in a siberian population of the Japanese scallop, Patinopecten yessoenssis (Jay).
J Shell Res,1997,16(2):541-545
|
被引
8
次
|
|
|
|
|
了解气象卫星更多信息，请访问