茄腐镰孢菌分泌蛋白与效应子的预测分析
Genome-wide prediction and analysis of secreted proteins and effectors in Fusarium solani
查看参考文献57篇
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文摘
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茄腐镰孢菌(Fusarium solani)寄主范围广泛,可引发生产上的毁灭性病害-根腐病。分泌蛋白在病原菌对植物的侵入、定殖和扩展等致病过程中起着重要作用,本研究利用SignalP、TMHMM、WoLF PSORT和PredGPI等软件对F.solani全基因组编码的17654条蛋白序列进行了分泌蛋白和效应子的预测分析,结果表明:F.solani基因组中有1032个分泌蛋白基因,占编码蛋白基因总数的5.85%。进一步利用dbCAN3等软件对经典分泌蛋白中的碳水化合物活性酶(Carbohydrate-active enzymes,CAZymes)进行预测,发现其中258个为CAZymes,以糖苷水解酶亚家族成员最多。分泌蛋白中效应蛋白(effector)的预测结果表明,F.solani中有185个潜在的效应蛋白,其中183个蛋白的功能可被PHI数据库注释到。利用农杆菌介导的植物瞬时表达系统,在本氏烟中对其中5个注释为增加F.solani毒力的效应蛋白进行验证,发现其中2个候选效应蛋白(XP_046140852.1和XP_046131041.1)能够抑制由Bcl2关联X蛋白BAX(BCL2-associated X protein)引起的细胞程序性死亡,可能在F.solani侵染致病过程中起重要作用。研究结果有助于揭示F.solani的致病分子机制,同时为F.solani与植物互作的深入研究提供了理论依据。 |
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其他语种文摘
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Fusarium solani,known for its extensive host range,is the causal agent of the destructive root rot disease in agriculture production.Secreted proteins play important roles in the infection of host plants by phytopathogenic fungi.To identify the secreted proteins and effectors in F.solani,we performed an in-depth analysis of the F.solani genome in this study.Among the total 17654 genomic proteins,1032 proteins were predicted to be the candidate secreted proteins by using SignalP,TMHMM,WoLF PSORT and PredGPI softwares,accounting for 5.85% of the total proteins in F.solani.Among them,258 proteins were predicated to be carbohydrate-active enzymes (CAZymes) by using the dbCAN3 software,with the glycoside hydrolase family being the most abundant.Furthermore,185 secreted proteins were predicated to be candidate effectors,with 183 sequences being annotated in the PHI database.By employing a virus-based transient expression system,we investigated the effect of the 5 candidate effectors annotated for increased virulence on BAX-triggered programmed cell death,and the result showed that the two effectors (XP_046140852.1 and XP_046131041.1) could suppress BAX-triggered programmed cell death in N.benthamiana.These findings provide not only an important reference for further analysis of the pathogenic molecular mechanism of F.solani but also a theoretical basis for understanding the interactions between F.solani and host plants. |
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来源
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植物病理学报
,2025,55(1):32-44 【核心库】
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DOI
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10.13926/j.cnki.apps.001646
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关键词
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茄腐镰孢菌
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生物信息学
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分泌蛋白
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效应蛋白
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碳水化合物酶类
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地址
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1.
浙江师范大学生命科学学院, 金华, 321000
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浙江省农业科学院蔬菜所, 杭州, 310000
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0412-0914 |
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学科
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植物保护 |
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基金
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浙江省自然科学基金
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浙江省"十四五"农业新品种选育重大科技专项
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浙江省"三农九方"农业科技协作项目
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文献收藏号
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CSCD:7901721
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