东北地区人参生炭疽菌对苯醚甲环唑和戊唑醇的敏感性及田间抗性监测
Sensitivity and resistance monitoring of Colletotrichum panacicola to difenoconazole and tebuconazole in Northeast China
查看参考文献29篇
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文摘
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为明确东北地区人参生炭疽菌(Colletotrichum panacicola)对苯醚甲环唑和戊唑醇的敏感性,采用菌丝生长速率法测定来自吉林、辽宁和黑龙江省109个C. panacicola菌株对这两种药剂的敏感性,并通过最低浓度抑制法评价吉林和辽宁省15个乡镇人参主产区435个C. panacicola菌株的抗性水平。结果表明,测试的109个C. panacicola菌株对苯醚甲环唑和戊唑醇的敏感性频率均符合正态分布,其菌株群体的EC_(50)均值分别为0.4044±0.1996 μg·mL~(-1)和0.1717±0.0045 μg·mL~(-1),可作为C. panacicola对苯醚甲环唑和戊唑醇的相对敏感性基线。不同地区来源的C. panacicola菌株对苯醚甲环唑和戊唑醇的敏感性无显著差异。进一步对109个C. panacicola菌株的log_(10) EC_(50)进行Spearman相关分析,发现这些菌株对苯醚甲环唑和戊唑醇的抗性无相关性。本研究发现了1个苯醚甲环唑低抗菌株,其抗性频率为0.22%;未监测到戊唑醇抗性菌株。表明苯醚甲环唑和戊唑醇对东北地区的C. panacicola抑菌活性较高,可继续用于防治人参炭疽病。研究结果为评估C. panacicola的抗药性风险奠定了基础,同时也为东北地区人参炭疽病的合理用药提供了科学依据。 |
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其他语种文摘
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In this study, the sensitivity of Colletotrichum panacicola to difenoconazole and tebuconazole was determined with 109 isolates collected from the main ginseng-growing regions in Jilin, Liaoning and Heilongjiang provinces by measuring mycelial growth rate in vitro. Meanwhile, the resistance level of 435 C. panacicola isolates from 15 villages and towns in Jilin and Liaoning provinces was evaluated via the minimum concentration inhibition method. The result showed that the sensitivity frequency of the 109 isolates of C. panacicala to difenoconazole and tebuconazole presented a normal distribution, with the average EC_(50) values of 0.4044 ±0.1996 μg·mL~(-1)and 0.1717±0.0045 μg·mL~(-1), respectively, which could be adopted as the relative susceptible baselines of C. panacicola to these two fungicides. Moreover, C. panacicola strains from different regions displayed no significant difference in the sensitivity to difenoconazole and tebuconazole. Spearman correlation analysis of log_(10) EC_(50) values of the 109 isolates of C. panacicola showed no cross resistance between the two fungicides. Here, only one strain of C. panacicola showed low resistance to difenoconazole, with a resistance frequency of 0.22%; no tebuconazole-resistant isolate was detected. In conclusion, the C. panacicala populations from Northeast China exhibited high sensitivity to difenoconazole and tebuconazole, indicating that the two fungicides can still be used for the control of anthracnose of ginseng. The results lay a basis for the assessment of resistance risk of C. panacicala to fungicides and also for the rational application of fungicides in the chemical control of ginseng anthracnose in Northeast China. |
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来源
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植物病理学报
,2024,54(2):419-428 【核心库】
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DOI
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10.13926/j.cnki.apps.001604
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关键词
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人参生炭疽菌
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苯醚甲环唑
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戊唑醇
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相对敏感性基线
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抗性监测
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地址
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吉林农业大学植物保护学院, 长春, 130118
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0412-0914 |
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学科
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植物保护 |
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基金
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国家中药材产业技术体系项目
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国家教育部高等学校学科创新引智计划项目
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国家自然科学基金国家杰出青年科学基金
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吉林省科技厅项目
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文献收藏号
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CSCD:7863505
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