金森女贞叶斑病病原物的鉴定、生物学特性及防治药剂筛选
Identification, characterization and fungicide screening of the pathogen causing leaf spot on Ligustrum japonicum
查看参考文献39篇
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文摘
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金森女贞叶斑病每年春季在扬州大学文汇路校区均普遍发生。为明确其病原种类及病菌生物学特性,筛选适于防治该病的化学药剂,作者对病原菌进行分离纯化、室内外离体与活体致病性测定、形态与多基因(ITS、GAPDH、RPB2和TEF1)序列分子鉴定,以及病原菌生物学特性和对5种杀菌剂的敏感性测定。结果表明,金森女贞叶斑病病原物为链格孢(Alternaria alternata),其最适生长条件为:以蔗糖或果糖为碳源、以硝酸钾为氮源,在25 ℃ 、pH 9.0条件下生长。5种杀菌剂对A. alternata均表现较强的毒力,其中嘧菌酯毒力最强,EC_(50)值为0.080 6 μg·mL~(-1);咪鲜胺、吡唑醚菌酯和戊唑醇次之, EC_(50)值分别为2.272 2 μg·mL~(-1) 、3.934 9 μg·mL~(-1)和6.400 0 μg·mL~(-1);而苯醚甲环唑的毒力最弱,EC_(50)值为15.486 0 μg·mL~(-1),说明这些药剂均可用于生产上金森女贞叶斑病的防控。 |
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其他语种文摘
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Ligustrum japonicum leaf spot occurs universally in Wenhuilu campus of Yangzhou university, Yangzhou, Jiangsu, China every spring. The aim of this study was to identify the pathogen species, determine the biological characteristics, and screen out effective pesticides for the prevention and control of the disease.The multiple assays were conducted for the pathogen identification including tissue separation and single-spore purification, pathogenicity test both in vitro and vivo to fulfill Koch's postulates, and morphological and phylogenetic analysis based on a combined ITS, GAPDH, RPB2 and TEF1 sequence dataset, the biological characterization, and the sensitivity test to the five fungicides. We obtained the purified fungal culture which was further identified as Alternaria alternata, a new pathogen causing leaf spot on L. japonicum. The effect of various carbon and nitrogen sources for growing of the representative isolate was investigated and A. alternata isolate was suitable for growing on the optimized medium supplied with sucrose, fructose, and potassium nitrate at 25 ℃, pH 9.0. In addition, 5 fungicides were assessed on the inhibitory effect against mycelial growth of A. alternata isolate in which azoxystrobin displayed the strongest inhibitory activity with EC_(50) value of 0.080 6 μg·mL~(-1), followed byprochloraz, pyraclostrobin and tebuconazole with EC_(50) values of 2.272 2 μg·mL~(-1), 3.934 9 μg·mL~(-1) and 6.400 0 μg·mL~(-1), respectively, while difenoconazole exhibited the least sensitivity at EC_(50) value of 15.486 0 μg·mL~(-1). These results indicated that these fungicides could be used for the prevention and control of L. japonicum leaf spot disease. |
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来源
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植物病理学报
,2024,54(1):36-48 【核心库】
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DOI
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10.13926/j.cnki.apps.001024
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关键词
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病原鉴定
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生物学特性
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药剂筛选
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链格孢
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金森女贞叶斑病
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地址
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1.
扬州大学植物保护学院, 江苏, 扬州, 225009
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教育部农业与农产品安全国际合作联合实验室, 教育部农业与农产品安全国际合作联合实验室, 江苏, 扬州, 225009
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江苏省农药总站, 江苏, 南京, 210036
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0412-0914 |
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学科
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植物保护 |
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基金
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江苏省现代农业产业技术体系
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扬州大学大学生科技创新项目
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文献收藏号
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CSCD:7679072
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