花椒枝枯病的病原鉴定与防治药剂筛选
Pathogen Identification and effective fungicides screening of branch blight of Zanthoxylum bungeanum
查看参考文献21篇
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文摘
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近年来,花椒枝枯病在陕西省花椒产区大面积地发生,严重影响了花椒的生长,导致花椒产量和质量的显著降低。为明确花椒枝枯病的病原,本研究通过组织分离法和柯赫氏法则致病性验证,分离获得花椒枝枯病菌。通过组织切片观察、形态学特征及ITS -β-tubulin-TEF1-α序列的串联分析,将花椒枝枯病菌鉴定为间座壳属真菌Diaporthe eres。杀菌剂室内毒力测定结果表明,供试的15种杀菌剂对花椒枝枯病菌的菌落生长均表现出不同程度的抑制作用,生物源杀菌剂中以3%多抗霉素可湿性粉剂和0.3%苦参碱乳油抑菌作用最显著,EC_(50)分别为0.046和0.608 mg·L~(-1);化学杀菌剂中以复配农药的抑菌作用更显著,以30%唑醚·戊唑醇悬浮剂、30%戊唑·多菌灵悬浮剂和12%腈菌·三唑桐乳油的抑菌作用最显著,EC_(50)分别为0.002、0.115和0.125 mg·L~(-1)。本研究能为花椒枝枯病的有效防控提供科学指导依据。 |
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其他语种文摘
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In recent years,branch blight has occurred in a large area in Zanthoxylum bungeanum cultivation regions of Shaanxi province,and seriously affects the growth,yield and quality of Z. bungeanum. To identify the pathogen causing branch blight of Z. bungeanum,tissue separation method and Koch's postulate were applied to obtain the pathogen of Z. bungeanum branch blight. It was then identified as Diaporthe eres by histologic sections, morphological characteristics and the sequences of ITS,β-tubulin and TEF1-α. The in vitro toxicity tests of fungicides against D. eres showed all the tested fifteen fungicides exhibited inhibitory effects on the colony growth of D. eres with different degrees. Among the biogenic fungicides,3% Polyoxin WP and 0.3% Matrine EC showed the strongest inhibitory effects with EC_(50) values of 0.046 and 0.608 mg·L~(-1),respectively. Among the chemical fungicides,the compound fungicides showed relatively stronger inhibitory effects,and 30% Pyraclostrobin ·Tebuconazole SC,30% Tebuconazole·Carbendazim SC and 12% Myclobutanil·Triadimefon exhibited the most obvious inhibitory effects with EC_(50) values of 0.002,0.115 mg·L~(-1) and 0.125 mg·L~(-1),respectively. The present study can provide scientific guidance for the effective control of Z. bungeanum branch blight. |
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来源
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植物病理学报
,2023,53(6):1056-1064 【核心库】
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DOI
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10.13926/j.cnki.apps.001309
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关键词
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花椒枝枯病
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形态鉴定
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分子鉴定
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间座壳属
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杀菌剂筛选
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地址
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1.
西北农林科技大学林学院, 西部森林生物灾害治理国家林业和草原局重点试验室, 杨凌, 712100
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陕西省林业科学院, 西安, 710082
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0412-0914 |
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学科
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林业 |
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基金
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陕西省重点研发计划
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全国大学生科研创新项目
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西北农林科技大学科技创新与成果转化项目
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文献收藏号
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CSCD:7645754
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